Microbial communities in co-digestion of food wastes and wastewater biosolids

The effect of food waste (FW) co-digestion with wastewater biosolids (WWB) on microbial communities was investigated through running thirteen lab-scale digesters for 100 days at different operational conditions i.e. organic loading rates (2 and 4 kgCOD/m³·day), feed types (WWB and FW), and FW content (10%, 90%, 100%). Compared with mono-digestion of WWB, FW co-digestion enhanced biogas production by 13% and COD degradation rates by up to 101%. Among fermentative bacteria/acetogens, Syntrophomonas was the dominant genus in FW digesters in contrast to the dominance of Clostridium in WWB digesters. The predominant methanogen was Methanosarcina in FW digesters in contrast to Methanosaeta in WWB digesters. COD degradation rates and methane yields were well correlated with Bacteroidetes population. Methane production rate was well correlated with Clostridium for FW digesters, with syntrophs for WWB digesters, and with aceticlastic methanogens for both digesters. Synergism was associated with hydrolytic bacteria, Clostridium, Syntrophomonas, syntrophs, Methanosarcina, and Methanobacterium.