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LncRNA MIR155HG regulates M1/M2 macrophage polarization in chronic obstructive pulmonary disease

Nannan Li1, Yuan Liu2, Jingfen Cai1

  • 1Department of Common Health, The Affiliated Wuxi Matemity and Child Health Care Hospital of Nanjing Medical University, No.48 Huaishu Lane, Liangxi District, Wuxi, Jiangsu 214002, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|June 18, 2019
PubMed
Abstract

Insights

The long non-coding RNA MIR155 host gene (MIR155HG) promotes M1 macrophage polarization in chronic obstructive pulmonary disease (COPD). MIR155HG modulation impacts M1/M2 macrophage balance, suggesting a role in COPD pathogenesis.

Area of Science:

  • Immunology
  • Molecular Biology
  • Respiratory Medicine

Background:

  • Macrophages are key players in inflammatory diseases like chronic obstructive pulmonary disease (COPD).
  • Long non-coding RNAs (lncRNAs) are emerging as regulators of macrophage function.
  • MIR155 host gene (MIR155HG) is a novel lncRNA implicated in macrophage polarization.

Purpose of the Study:

  • To investigate the role of MIR155HG in the context of COPD.
  • To determine MIR155HG's effect on macrophage polarization in COPD patients.

Main Methods:

  • Peripheral blood mononuclear cells (PBMCs) from COPD patients and controls were differentiated into macrophages using granulocyte-macrophage colony-stimulating factor (GM-CSF).
  • Quantitative real-time PCR (QRT-PCR) and flow cytometry were used to assess MIR155HG expression and M1/M2 macrophage populations.
  • Enzyme-linked immunosorbent assay (ELISA) measured inflammatory cytokine levels.

Main Results:

  • MIR155HG expression was significantly higher in GM-CSF-induced macrophages from COPD patients.
  • Overexpression of MIR155HG enhanced M1 macrophage polarization and pro-inflammatory cytokine release.
  • Knockdown of MIR155HG inhibited M1 polarization and promoted M2 macrophage polarization.

Conclusions:

  • LncRNA MIR155HG plays a critical role in modulating GM-CSF-mediated M1/M2 macrophage polarization.
  • MIR155HG influences COPD progression through its effects on macrophage phenotype.
  • Targeting MIR155HG may offer a therapeutic strategy for COPD.
Keywords:
Chronic obstructive pulmonary diseaseMIR155HGMacrophage polarization

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