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Immunostick Colorimetric Assay for Detection of Pseudomonas spp. in Refrigerated Meat and Milk
Rosalba Gutiérrez1, Teresa García1, Isabel González1
1Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
Abstract:
Monoclonal antibodies generated against live cells of Pseudomonas fluorescens have been used in an immunostick enzyme-linked immunosorbent assay (ELISA) format for the detection of Pseudomonas spp. in refrigerated meat and milk. The detection threshold for the immunostick ELISA assay developed in this work is 104 CPU cm-2 for meat and 105 CPU ml-1 for milk samples.
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