Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Genetic Engineering?00:49

What is Genetic Engineering?

79.8K
Overview
79.8K
Therapeutic Index01:13

Therapeutic Index

6.7K
The therapeutic index of a drug is a key parameter in pharmacology that quantifies the relative safety of a drug by calculating the ratio between the dose that causes toxicity in half the population (50%) to the dose that proves to be effective for half the population (50%). It provides a spectrum of doses for a particular drug ranging from effective to potentially toxic. To illustrate, consider an anticoagulant agent like warfarin. It possesses a narrow window within its therapeutic index to...
6.7K
Therapeutic Communication01:30

Therapeutic Communication

7.7K
Communication is a lifelong learning process. Through therapeutic communication, nurses can collect relevant assessment data, provide education and counseling, and interact during nursing interventions. Sending and receiving messages occur through verbal and nonverbal communication techniques and can happen separately or simultaneously.
Verbal communication depends on language or a prescribed way of using words so that people can share information effectively. The critical aspects of verbal...
7.7K
Impression Management Techniques I: Managing Appearances01:29

Impression Management Techniques I: Managing Appearances

205
Appearance is a multidimensional aspect of self-presentation that encompasses observable attributes such as clothing, grooming, speech, and nonverbal behavior. These elements are often strategically managed to align with socially constructed expectations in different settings. For instance, individuals tailor their appearance during job interviews, social gatherings, or athletic events to meet the perceived norms of those environments.Contextual Adaptation and Social SignalsThe research...
205
Tonsillitis II: Management01:26

Tonsillitis II: Management

412
This lesson will focus on the different treatment options for managing tonsillitis, which typically depend on the cause and severity.
412
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

1.0K
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
1.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The ITCC-P4 PDX Platform Enables Preclinical Testing of Pediatric Cancers.

Cancer research·2026
Same author

Completion Cholecystectomy: Indications and Outcomes After Subtotal Cholecystectomy.

Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract·2026
Same author

Institutionalizing grant-funded interventions: a multiple case study examining long-term investments in science, technology, engineering, mathematics, and medicine (STEMM).

International journal of STEM education·2026
Same author

Fully human anti-FOLR1 T-cell engager demonstrates potent activity in CBFA2T3::GLIS2 acute megakaryoblastic leukemia.

Blood neoplasia·2026
Same author

Interaction of spirituality with entrepreneurial attitudes and intentions in South Korea: extending the theory of planned behavior.

Frontiers in psychology·2026
Same author

Cervical Spine Musculoskeletal Work-Related Injuries in Microsurgery: Insights of an Often-Overlooked Aspect of Microsurgical Practice.

Microsurgery·2026

Related Experiment Video

Updated: Jan 23, 2026

Author Spotlight: Workflow for Integrating POCUS Data into EHR for Managing Heart Failure Patients
03:47

Author Spotlight: Workflow for Integrating POCUS Data into EHR for Managing Heart Failure Patients

Published on: July 12, 2024

1.1K

Laboratory information management software for engineered mini-protein therapeutic workflow.

Mi-Youn Brusniak1, Hector Ramos2, Bernard Lee3

  • 1Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, Seattle, WA, 98109, USA. mbrusnia@fredhutch.org.

BMC Bioinformatics
|June 19, 2019
PubMed
Summary

A new laboratory information management system (LIMS), Optide-Hunter, tracks engineered protein therapeutics from creation to preclinical studies. This open-source system aids multidisciplinary labs in optimizing therapeutic compounds and protein science applications.

Keywords:
HPLC/UPLC peak classificationLabKey software™Laboratory information management systemProtein engineeringTherapeutic protein

More Related Videos

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
10:23

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

Published on: June 23, 2023

3.5K
Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

3.4K

Related Experiment Videos

Last Updated: Jan 23, 2026

Author Spotlight: Workflow for Integrating POCUS Data into EHR for Managing Heart Failure Patients
03:47

Author Spotlight: Workflow for Integrating POCUS Data into EHR for Managing Heart Failure Patients

Published on: July 12, 2024

1.1K
Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
10:23

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

Published on: June 23, 2023

3.5K
Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

3.4K

Area of Science:

  • Biotechnology
  • Pharmaceutical Sciences
  • Bioinformatics

Background:

  • Protein-based therapeutics are a rapidly growing class of medical interventions for cancer, infectious diseases, and inflammation.
  • Protein engineering optimizes therapeutic properties like stability, specificity, and reduced immunogenicity.
  • Engineered proteins, such as cystine-dense peptides (CDPs) and optimized peptides (Optides), are often modified via synthetic chemistry for therapeutic applications.

Purpose of the Study:

  • To develop a cost-effective and extendable laboratory information management system (LIMS).
  • To support multidisciplinary lab scientists in non-commercial settings with diverse therapeutic portfolio research goals.
  • To efficiently manage the workflow for engineered protein compounds from creation to preclinical experiments.

Main Methods:

  • Developed Optide-Hunter LIMS using the Open Source LabKey server platform.
  • Integrated compound registry, in-silico and in-vivo assays, and production tracking.
  • Incorporated automated chromatogram classification via HPLCPeakClassifierApp for HPLC data pre-processing.
  • Customized implementation using R, SQL, JavaScript, and Java data transformation modules.

Main Results:

  • Optide-Hunter system successfully tracks engineered protein compounds and assays throughout the research workflow.
  • The system facilitates storage, extraction, and visualization of data for various therapeutics projects.
  • Included HPLCPeakClassifierApp for automated HPLC data pre-processing, enhancing data quality.
  • The system is built on an Open Source platform, allowing for customization and extension.

Conclusions:

  • Optide-Hunter LIMS effectively tracks the engineering, production, and prioritization of protein therapeutic candidates.
  • The system is adaptable for both small and large research laboratories with collaborative, multidisciplinary teams.
  • Open Source exploration enables bioinformatics scientists to tailor and deploy similar systems for specific laboratory workflows.