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Related Concept Videos

Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

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Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
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Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
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Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
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Protein Complexes with Interchangeable Parts01:57

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Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
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A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
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In vivo and in vitro Studies of Adaptor-clathrin Interaction
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In vivo and in vitro Studies of Adaptor-clathrin Interaction

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Clathrin-containing adhesion complexes.

John G Lock1, Francesco Baschieri2, Matthew C Jones3

  • 1Department of Pathology, School of Medical Sciences, University of New South Wales, Sydney, Australia.

The Journal of Cell Biology
|June 19, 2019
PubMed
Summary
This summary is machine-generated.

Researchers discovered a new class of cell adhesion complexes (ACs) that lack cytoskeleton connections but involve clathrin-mediated endocytosis machinery. This finding reveals a novel link between cell adhesion and clathrin structures.

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Area of Science:

  • Cell Biology
  • Molecular Biology

Background:

  • Cell adhesion complexes (ACs) are crucial for cell signaling, migration, differentiation, and growth.
  • Existing ACs typically associate with the actin cytoskeleton and integrin-associated proteins.
  • The mechanisms of bidirectional signaling across the plasma membrane via ACs require further elucidation.

Purpose of the Study:

  • To characterize a newly identified class of cell adhesion structures.
  • To investigate the relationship between this new AC type and cellular processes.
  • To highlight the connection between adhesion machinery and clathrin-mediated endocytosis.

Main Methods:

  • Characterization of novel adhesion structures in mitotic and long-term cultured cells.
  • Analysis of AC composition, focusing on cytoskeletal and clathrin machinery components.
  • Investigating the association of these ACs with integrin-associated adhesome components.

Main Results:

  • A novel class of ACs was identified, distinct from previously known types.
  • These new ACs lack direct association with the actin cytoskeleton.
  • The novel ACs are enriched in components of the clathrin-mediated endocytosis machinery.

Conclusions:

  • A previously underappreciated link exists between cell adhesion complexes and clathrin structures.
  • This discovery impacts our understanding of AC assembly and disassembly.
  • Further research is needed to explore the implications of this new class of ACs.