Related Experiment Video

Updated: Jan 23, 2026

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
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Published on: October 18, 2013

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KRAS Exon 3 and PTEN Exon 7 Mutations in Small-cell Lung Cancer

Lei Lei1,2, Zhi-Ming Jiang1, Cheng-Hui Li2

  • 1Zhejiang Key Laboratory of Diagnosis & Treatment Technology on Thoracic Oncology (Lung and Esophagus), Zhejiang Cancer Hospital, Hangzhou, 310022, China.

Current Medical Science
|June 19, 2019
PubMed

Insights

Investigating KRAS and PTEN mutations in small cell lung cancer (SCLC) revealed no significant impact on patient survival. Extensive disease stage remains the primary negative prognostic factor for overall survival in SCLC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Small cell lung cancer (SCLC) is a highly aggressive malignancy with limited therapeutic advancements.
  • Identifying novel therapeutic targets is crucial for improving overall survival (OS) in SCLC patients.

Purpose of the Study:

  • To investigate the mutation status of K-Ras (KRAS) exon 3 and phosphatase and tensin homolog (PTEN) exon 7 in SCLC.
  • To determine the association between these mutations and the overall survival (OS) of SCLC patients.

Main Methods:

  • Direct sequencing of KRAS exon 3 and PTEN exon 7 in 19 primary SCLC tumor specimens.
  • Kaplan-Meier and Cox regression analyses were used to assess the prognostic significance of mutations and clinical factors.

Main Results:

  • KRAS exon 3 mutations were detected in 21% of SCLC patients, and PTEN exon 7 mutations in 5%.
  • Neither KRAS exon 3 nor PTEN exon 7 mutations were significantly associated with OS.
  • Extensive stage of disease was identified as the sole independent negative prognostic factor for OS.

Conclusions:

  • KRAS exon 3 and PTEN exon 7 mutations do not appear to significantly impact OS in SCLC patients.
  • Further research is warranted to validate these molecular profiles and explore other potential therapeutic targets in SCLC.
Keywords:
Rasgeneprognosissmall cell lung carcinoma

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