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Updated: Jan 23, 2026

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
12:03

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma

Published on: July 25, 2011

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Long noncoding RNA CASC2 alleviates the growth, migration and invasion of oral squamous cell carcinoma via

H-B Xing1, H-M Qiu, Y Li

  • 1Department of Stomatology, Weihai Municipal Hospital, Weihai, China. meimei_zhu0602@163.com.

European Review for Medical and Pharmacological Sciences
|June 19, 2019
PubMed
Abstract

Insights

Long noncoding RNA CASC2 suppresses oral squamous cell carcinoma (OSCC) progression by downregulating CDK1. This finding offers a potential new therapeutic strategy for OSCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Long noncoding RNAs (lncRNAs) play a critical role in tumorigenesis.
  • Oral squamous cell carcinoma (OSCC) is a globally prevalent cancer.
  • Understanding the specific mechanisms of lncRNAs in OSCC is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the role of lncRNA CASC2 in oral squamous cell carcinoma (OSCC).
  • To elucidate the underlying molecular mechanism by which CASC2 affects OSCC progression.
  • To explore the potential of CASC2 as a therapeutic target for OSCC.

Main Methods:

  • Quantitative Real-time polymerase chain reaction (qRT-PCR) to detect CASC2 expression in OSCC cell lines and tissues.
  • In vitro assays (Transwell, wound healing, proliferation) to assess the functional impact of CASC2 on OSCC cell behavior.
  • qRT-PCR and Western blot to explore the mechanism of CASC2 action, focusing on CDK1 regulation.

Main Results:

  • CASC2 expression was significantly downregulated in OSCC tissues compared to adjacent normal samples.
  • Overexpression of CASC2 inhibited proliferation, invasion, and migration of OSCC cells in vitro.
  • CASC2 overexpression led to the downregulation of CDK1 at both mRNA and protein levels in OSCC cells.
  • A negative correlation was observed between CASC2 and CDK1 expression in OSCC tissues.

Conclusions:

  • lncRNA CASC2 acts as a tumor suppressor in OSCC by inhibiting cell migration, invasion, and proliferation.
  • CASC2 exerts its suppressive effects through the downregulation of CDK1.
  • Targeting CASC2 may represent a novel therapeutic strategy for oral squamous cell carcinoma.

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