Reduced Carboxylate Graphene Oxide based Field Effect Transistor as Pb2+ Aptamer Sensor
Fang Li1, Zhongrong Wang2, Yunfang Jia3
1College of Electronic Information and Optical Engineering, Nankai University, Tianjin 300071, China. lifang_6952@163.com.
Micromachines
|June 20, 2019
Summary
Reduced carboxyl-graphene oxide (rGO-COOH) enhances aptamer functionalized graphene field-effect transistors (apta-GFETs). This novel material improves biosensing capabilities for lead detection, overcoming graphene
Area of Science:
- Materials Science
- Nanotechnology
- Biosensors
Background:
- Aptamer functionalized graphene field-effect transistors (apta-GFETs) offer versatile biosensing but face challenges due to graphene's chemical inertness.
- Commercialization and large-scale application of apta-GFETs are hindered by limitations in material functionalization and probe immobilization.
Purpose of the Study:
- To investigate reduced carboxyl-graphene oxide (rGO-COOH) as a self-activated channel material for screen-printed apta-GFETs.
- To demonstrate the efficacy of rGO-COOH in accommodating aptamer bio-probes and enhancing sensing reactions, using lead detection as a model.
Main Methods:
- Characterization of rGO-COOH films using X-ray photoelectron spectroscopy, Raman spectroscopy, UV-visible absorbance, atomic force microscopy, and scanning electron microscopy.
- Development of a site-binding model based on the solution-gated field-effect transistor (SgFET) working principle to explain enhanced responses.
- Testing specificity with disturbing ions and real samples, and comparison with inductively coupled plasma mass spectrometry (ICP-MS).
Main Results:
- rGO-COOH films exhibit a few-layer nanostructure with abundant oxygen-containing groups, facilitating enhanced aptamer immobilization.
- The rGO-COOH channeled apta-GFET demonstrated high specificity, a low limit-of-detection (0.001 ppb) for lead, and results consistent with ICP-MS.
- Theoretical deductions supported the enhanced performance of rGO-COOH based apta-GFETs.
Conclusions:
- rGO-COOH is a promising self-activated channel material for apta-GFETs, independent of linking reagents and free from polymer residue.
- The material's compatibility with print-electronic technology facilitates large-scale applications and commercialization of advanced biosensors.
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