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Updated: Jun 25, 2026

Extraction and Analysis of Cortisol from Human and Monkey Hair
Published on: January 24, 2014
Cortisol, DHEA, and Sexual Steroid Concentrations in Fattening Pigs' Hair
Cristina Bergamin1, Antonella Comin2, Mirco Corazzin3
1Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, 33100 Udine, Italy. bergamin.cristina@spes.uniud.it.
Abstract:
The aim of this study was to analyze the feasibility and reliability of using hair as a matrix to determine the dehydroepiandrosterone (DHEA) and sexual steroid concentrations and the cortisol/DHEA ratio in fattening pigs. The results could be also used to plan future research to identify threshold values in order to set up strategies to control the allostatic load and increase the resilience of fattening pigs before slaughter. The study was conducted on 107 commercial crossbred rearing pigs. The hair samples were taken by shaving at the age of 36 weeks, and concentrations of the hormones were measured using a solid-phase microtiter radioimmunoassay. Females had significantly higher cortisol levels (p < 0.01), significantly lower DHEA concentrations (p < 0.05) and significantly higher cortisol/DHEA ratios (p < 0.01) than barrows. Progesterone was significantly higher in gilts than in barrows (p < 0.01). Testosterone and 17β-estradiol were significantly higher in barrows than in gilts (p < 0.05). If future research can produce threshold values for the different markers examined, the evaluation of animals under subclinical stress conditions will be possible.
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