Related Experiment Video
Updated: Jan 23, 2026
09:48
An Orthotopic Endometrial Cancer Model with Retroperitoneal Lymphadenopathy Made From In Vivo Propagated and Cultured VX2 Cells
Published on: September 12, 2019
8.6K
Optimization of Window Study Endpoints in Endometrial Cancer
Sarah J Kitson1, Zoe Maskell1, Vanitha N Sivalingam1
1Division of Cancer Sciences, Faculty of Biology, Medicine and Health, University of Manchester, St. Mary's Hospital, Manchester, United Kingdom.
Frontiers in Oncology
|June 20, 2019
Summary
Endometrial cancer biomarker expression differs between pre-surgical biopsies and hysterectomy specimens. Prompt tissue fixation is critical for accurate analysis in window studies.
Area of Science:
- Oncology
- Pathology
- Biomarker Research
Background:
- Pre-surgical window studies in endometrial cancer assess treatment response using biomarker quantification.
- Traditionally, immunohistochemistry compares diagnostic biopsies with post-treatment hysterectomy specimens.
- This method risks inaccurate results due to surgical hypoxia or delayed tissue fixation causing protein loss.
Purpose of the Study:
- To compare immunohistochemical biomarker expression in pre-operative endometrial biopsies versus same-day hysterectomy specimens.
- To correlate expression differences with clinico-pathological variables and tissue handling.
- To evaluate the impact of tissue handling on biomarker preservation.
Main Methods:
- Compared immunohistochemical expression of Ki-67, PI3K-Akt-mTOR pathway markers, insulin signaling markers, hormone receptors, CD133, and ALDH.
- Analyzed specimens from 75 endometrial cancer patients in a clinical trial.
- Correlated expression differences with clinico-pathological data and assessed the effect of uterine bisection before formalin fixation.
Main Results:
- Significantly lower expression of Ki-67, PI3K-Akt-mTOR, insulin signaling markers, and hormone receptors in hysterectomy specimens compared to biopsies (p < 0.0001).
- Similar expression of cancer stem cell markers CD133 and ALDH in both specimen types.
- Protein loss in hysterectomy specimens strongly correlated with baseline biopsy expression (p ≤ 0.001).
- Uterine bisection partially preserved protein expression, highlighting the importance of prompt fixation.
Conclusions:
- Hysterectomy specimens show reduced protein expression for key endometrial cancer biomarkers compared to pre-operative biopsies.
- Findings challenge previous endometrial cancer window studies relying solely on hysterectomy specimens.
- Recommend including post-intervention endometrial biopsies in future clinical trials for accurate biomarker analysis.
More Related Videos
05:52Sentinel Lymph Node Mapping and Biopsy for Endometrial Cancer at Early Stage with Laparoscopy
Published on: August 19, 2021
13.1K
07:44Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice
Published on: February 17, 2023
2.0K
Related Concept Videos
01:19Precipitation Titration: Endpoint Detection Methods
5.8K
In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
In the Volhard method, a standard excess of AgNO3 is first added to the...
In the Volhard method, a standard excess of AgNO3 is first added to the...
5.8K
02:43Mouse Models of Cancer Study
6.4K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
6.4K
00:48Optimal Foraging
13.7K
How animals obtain and eat their food is called foraging behavior. Foraging can include searching for plants and hunting for prey and depends on the species and environment.
13.7K
01:26Optimization Problems
55
Optimization problems often involve identifying maximum or minimum values under specific constraints. A well-known example is determining the longest horizontal pipe that can be moved around a right-angled corner, where a 3-meter-wide hallway meets a 2-meter-wide hallway. This scenario, common in architectural design and industrial transport, can be understood conceptually through geometric and trigonometric reasoning.To visualize the problem, consider the pipe as a straight line that touches...
55
01:23Optimal Arousal Theory
806
The optimal arousal theory suggests that performance is maximized when an individual experiences a moderate level of arousal. This theory is closely tied to the Yerkes-Dodson law, which illustrates an inverted U-shaped relationship between arousal and performance. The law, formulated by psychologists Robert Yerkes and John Dodson, implies an ideal arousal level for optimal performance, and deviations from this level can lead to declines in effectiveness.
Inverted U-Shaped Performance Curve
The...
Inverted U-Shaped Performance Curve
The...
806
01:30Unrealistic Optimism Bias
216
Unrealistic optimism bias is the tendency to overestimate the likelihood of positive outcomes. This cognitive bias makes individuals believe they are less likely to experience failures, setbacks, or risks and more likely to succeed than others. For example, people may assume they are less prone to health issues, accidents, or financial struggles than their peers, even when they share similar risk factors.One key component of this bias is the above-average effect, where individuals perceive...
216