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Updated: Jan 23, 2026

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
Published on: June 23, 2016
A dual-mode fluorometric/colorimetric sensor for Cu2+ detection based on hybridized carbon dots and gold-silver
Yuqing Chen1, Yawen Lian1, Mengna Huang1
1Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), Key Laboratory of Phytochemical R&D of Hunan Province, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China. happywei2012@126.com.
Abstract:
A fluorometric and colorimetric dual mode sensing platform based on hybridized carbon dots (Cdots) and gold-silver core-shell nanoparticles (Au@Ag NPs) has been established for the sensitive detection of trace Cu2+ ions in aqueous solution. In this system, the fluorescence of Cdots was quenched by Au@Ag NPs due to the surface plasmon-enhanced energy transfer. Due to the fact that Cu2+ could accelerate the etching process of Au@Ag NPs in the presence of thiosulfate, the fluorescence of Cdots was recovered. The limit of detection (LOD) is 4.81 nM for fluorometric measurements and 3.85 nM for colorimetric measurements. The dynamic range from these two modes is 0.005-1 μM. Importantly, Cu2+ in solution can also be directly visualized by this sensor via evident color change from the solution. Therefore, this dual mode nanosensor has potential applications for the efficient detection of Cu2+ ions in aqueous samples with great selectivity and high sensitivity.
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