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Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging
Published on: December 12, 2012
Two-Photon Absorption and Cell Imaging of Fluorene-Functionalized Epicocconone Analogues
Samuel Oger1, Damien Schapman2, Romain Mougeot1
1COBRA (UMR 6014 and FR 3038), Normandie Univ, CNRS, INSA Rouen, UNIROUEN, 76000, Rouen, France.
Abstract:
Epicocconone 1 is a natural chromophore isolated from the fungus Epicoccum nigrum that has shown applications in proteomics and fluorescent microscopy thanks to its unique pro-fluorescence properties. The modification of the skeleton of the natural product by replacing the triene side chain by a fluorenyl scaffold can noticeably increase the fluorophore's absorption coefficient. The synthesis of the analogues of the natural product has been made possible by the use of a palladium-catalyzed carbonylation reaction, allowing the construction of the β-keto-dioxinone key intermediate. Two-photon absorption cross-section measurements of the fluorenyl epicocconone analogues show a structure dependency with values ranging from 60 to 280 GM and live cell imaging show intense staining of intracellular vesicle-like structures around the nucleus.
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