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LIN28B regulates androgen receptor in human trophoblast cells through Let-7c

Erin S McWhorter1, Rachel C West1, Jennifer E Russ1

  • 1Department of Biomedical Sciences, Animal Reproduction and Biotechnology Laboratory, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado.

Molecular Reproduction and Development
|June 20, 2019
PubMed

Insights

LIN28B protein regulates androgen receptor (AR) levels in human trophoblast cells, likely via let-7c microRNA. This pathway may be crucial for trophoblast cell differentiation into syncytiotrophoblast.

Area of Science:

  • Reproductive Biology
  • Stem Cell Biology
  • Molecular Endocrinology

Background:

  • LIN28B is essential for stem cell pluripotency and trophoblast differentiation.
  • LIN28B interacts with the Let-7 miRNA family to regulate gene expression.
  • Androgen receptor (AR) regulation by LIN28B has been observed in cancer cells.

Purpose of the Study:

  • To investigate the role of LIN28B in regulating androgen receptor (AR) levels in human trophoblast cells.
  • To explore the potential involvement of the Let-7 miRNA family in this regulation.

Main Methods:

  • Utilized the human first-trimester trophoblast cell line ACH-3P.
  • Constructed a LIN28B knockdown cell line using lentiviral vectors.
  • Administered Let-7c mimic and forskolin to evaluate effects on AR and syncytiotrophoblast markers.

Main Results:

  • LIN28B knockdown led to decreased AR levels and increased Let-7 miRNAs.
  • Let-7c mimic treatment reduced both LIN28B and AR levels.
  • Forskolin-induced syncytialization and Let-7c treatment increased ERVW-1 expression and decreased AR levels.

Conclusions:

  • LIN28B regulates AR levels in trophoblast cells, potentially through inhibition of let-7c.
  • This LIN28B-let-7c-AR axis may be vital for human trophoblast cell differentiation into syncytiotrophoblast.
Keywords:
ARLIN28Blet-7 miRNAplacentatrophoblast cells

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