Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of Species01:31

Formation of Species

44.8K
Speciation describes the formation of one or more new species from one or sometimes multiple original species. The resulting species are discrete from the parent species, and barriers to reproduction will typically exist. There are two primary mechanisms, speciation with and without geographic isolation—allopatric and sympatric speciation, respectively.
44.8K
What is a Species?01:17

What is a Species?

49.5K
Overview
49.5K
Keystone Species01:39

Keystone Species

24.2K
Measures of species biodiversity, such as richness (i.e., the number of species present) and evenness (i.e., their relative abundance), describe an ecological community’s structure. Many factors affect community structure, including abiotic factors (e.g., sunlight and nutrients), disturbances (e.g., fire or flood), species interactions (e.g., predation or competition), and chance events (e.g., foreign species invasion). Certain species—such as keystone species—also play a...
24.2K
Protein Complex Assembly02:41

Protein Complex Assembly

16.7K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.7K
Assessment of blood pressure in brachial artery(one-step method)01:15

Assessment of blood pressure in brachial artery(one-step method)

1.1K
This procedural guide systematically measures blood pressure using an oscillometric digital sphygmomanometer, emphasizing accuracy, patient safety, and comfort.
Prepare for the Procedure:
1.1K
Assessment of blood pressure in brachial artery(two-step method)01:23

Assessment of blood pressure in brachial artery(two-step method)

1.5K
Measuring blood pressure is a fundamental skill in healthcare that aids in diagnosing and monitoring hypertension and other cardiovascular conditions. An aneroid sphygmomanometer, commonly used in clinical settings, offers a manual and precise method for blood pressure measurement. The technique for using this instrument involves specific steps that must be carefully executed to ensure accuracy. The following detailed description outlines a two-step technique for assessing blood pressure using...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cryo-SEM documentation of intact frozen-fractured avian eggs reveals species-dependent albumen freezing morphologies.

Micron (Oxford, England : 1993)·2026
Same author

Investigating the population dynamics of <i>Fusarium oxysporum</i> f. sp. <i>eustomae</i> causing stem rot in lisianthus with the PCR-based markers and virulence.

Plant disease·2026
Same author

Emergence of outbreak-driving high-risk <i>Pseudomonas aeruginosa</i> lineages in Taiwan: phylogenomic insights into ST292 and the ST235* sublineage.

Microbial genomics·2026
Same author

Orchid diseases caused by <i>Fusarium oxysporum</i> species complex in Taiwan.

Frontiers in plant science·2026
Same author

<i>Fusarium incarnatum</i>: a paradigm for One Health pathogen dynamics across humans, animals, and the environment.

Microbiology spectrum·2025
Same author

Gaps in the HIV self-testing cascade among people with HIV in Taiwan: Usage patterns, barriers, and public health implications.

Journal of infection and public health·2025

Related Experiment Video

Updated: Jan 23, 2026

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
08:54

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

15.9K

Two-step method for isolating Cryptococcus species complex from environmental material using a new selective medium.

Kuo-Hsi Lin1,2, Yi-Pei Lin2, Wen-Hsin Chung1,3

  • 1National Chung Hsing University, Taichung, Taiwan.

Environmental Microbiology Reports
|June 20, 2019
PubMed
Summary
This summary is machine-generated.

A new selective medium effectively isolates Cryptococcus yeast from environmental samples by inhibiting fungal overgrowth. This method aids in studying Cryptococcus species and their ecological roles.

More Related Videos

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
09:34

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species

Published on: November 8, 2016

15.4K
A Two-Step Protocol for Umpolung Functionalization of Ketones Via Enolonium Species
08:12

A Two-Step Protocol for Umpolung Functionalization of Ketones Via Enolonium Species

Published on: August 16, 2018

10.5K

Related Experiment Videos

Last Updated: Jan 23, 2026

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
08:54

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

15.9K
Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
09:34

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species

Published on: November 8, 2016

15.4K
A Two-Step Protocol for Umpolung Functionalization of Ketones Via Enolonium Species
08:12

A Two-Step Protocol for Umpolung Functionalization of Ketones Via Enolonium Species

Published on: August 16, 2018

10.5K

Area of Science:

  • Mycology
  • Environmental Microbiology
  • Infectious Diseases

Background:

  • Cryptococcosis, an opportunistic infection, is caused by the *Cryptococcus* species complex.
  • An outbreak of *Cryptococcus gattii* in North America highlights the need to study its environmental niche.
  • Isolating *Cryptococcus* from environmental samples is challenging due to filamentous fungal overgrowth and low cell density.

Purpose of the Study:

  • To develop a selective medium for isolating *Cryptococcus* species from environmental sources.
  • To overcome limitations in current methods for *Cryptococcus* isolation.

Main Methods:

  • Design of a selective culture medium to inhibit filamentous fungi while allowing *Cryptococcus* growth.
  • A two-step isolation process involving enrichment in acidified YPD media followed by inoculation onto the selective medium.
  • Incubation and observation of brown-colored colonies indicative of *Cryptococcus*.

Main Results:

  • The selective medium successfully inhibited the growth of common environmental filamentous fungi.
  • *Cryptococcus* cells were effectively isolated from environmental materials, appearing as brown colonies.
  • The two-step method demonstrated utility in isolating *Cryptococcus* from diverse environmental sources.

Conclusions:

  • A novel two-step method using selective media facilitates the isolation of *Cryptococcus* species from environmental samples.
  • This improved isolation technique is crucial for further research into *Cryptococcus* diversity and its environmental interactions.
  • The method addresses a significant bottleneck in the study of pathogenic yeasts like *Cryptococcus*.