Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The G protein beta 2 complementary DNA encodes the beta 35 subunit.

B Gao1, S Mumby, A G Gilman

  • 1Department of Pharmacology, Southwestern Graduate School, University of Texas Health Science Center at Dallas 75235.

The Journal of Biological Chemistry
|December 25, 1987
PubMed
Summary

Researchers generated specific antibodies to identify a new form of the beta subunit of guanine nucleotide-binding regulatory proteins (G proteins), termed beta 35. This discovery advances understanding of G protein signaling pathways.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transient Large-Scale Anisotropy in TeV Cosmic Rays due to an Interplanetary Coronal Mass Ejection.

Physical review letters·2026
Same author

First Detection of Ultrahigh Energy Emission from Gamma-Ray Binary LS I +61° 303.

Physical review letters·2026
Same author

Evidence of Cosmic-Ray Acceleration up to Sub-PeV Energies in the Supernova Remnant IC 443.

Physical review letters·2026
Same author

Precise Measurement of the Cosmic Ray Helium Spectrum above 0.1 PeV.

Physical review letters·2026
Same author

All-Sky Search for Individual Primordial Black Hole Bursts with LHAASO.

Physical review letters·2025
Same author

Rucaparib maintenance for newly diagnosed advanced ovarian cancer: interim overall survival, progression-free survival, and safety at 5 years of follow-up from the phase III ATHENA-MONO/GOG-3020/ENGOT-ov45 study.

Annals of oncology : official journal of the European Society for Medical Oncology·2025

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cellular Signaling

Background:

  • Guanine nucleotide-binding regulatory proteins (G proteins) are crucial signal transducers.
  • Multiple forms of G protein subunits exist, contributing to signaling diversity.
  • The beta subunit family includes several isoforms with distinct functions.

Purpose of the Study:

  • To characterize a novel cDNA sequence encoding a second form of the G protein beta subunit.
  • To generate specific antibodies for identifying and validating this new beta subunit form.
  • To confirm the expression of the identified beta subunit in cellular systems.

Main Methods:

  • Generation of antisera against synthetic peptides from a beta 2 cDNA sequence.
  • Testing antibody specificity against purified G protein beta subunits.

Related Experiment Videos

  • Detection of beta 2 cDNA expression in COS-m6 cells using generated antisera.

    • Main Results:

    • Antisera specifically recognized purified G protein beta subunits.
    • The beta 2 cDNA was confirmed to encode the beta 35 form of the G protein beta subunit.
    • Expression of the beta 2 cDNA was successfully detected in COS-m6 cells.

    Conclusions:

    • The beta 2 cDNA encodes the beta 35 G protein beta subunit.
    • Specific antisera are valuable tools for studying G protein beta subunit expression.
    • This finding expands the known repertoire of G protein beta subunits and their potential roles in signaling.