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Related Concept Videos

Development of Analytical Methods01:21

Development of Analytical Methods

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An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...
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Carl Jung, a Swiss psychiatrist and former follower of Freud, eventually broke away from Freud's ideas to create his framework, analytical psychology. This approach emphasizes achieving a balance between the conscious and unconscious aspects of the mind and reconciling various experiences within an individual's personality. Jung believed that this process, which typically unfolds in the latter part of life, involves an ongoing journey of recognizing and incorporating unconscious...
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Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
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Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo
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Analytical Methods for Quantitative Plasma Carnitine Determination.

Chelsea N Zimmerman1, J Daniel Sharer1

  • 1Department of Genetics, University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.

Current Protocols in Human Genetics
|June 20, 2019
PubMed
Summary
This summary is machine-generated.

Carnitine analysis in plasma and urine is crucial for diagnosing inherited metabolic disorders and secondary deficiencies. Quantitative methods assess fatty acid metabolism and carnitine transport, aiding clinical management.

Keywords:
carnitinemass spectrometryradioisotopespectrophotometry

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Area of Science:

  • Biochemistry
  • Metabolic Disorders
  • Clinical Chemistry

Background:

  • Carnitine is vital for mitochondrial beta-oxidation of long-chain fatty acids.
  • Inherited carnitine metabolism disorders and secondary deficiencies are clinically significant.
  • Carnitine analysis aids in diagnosing and managing these conditions.

Purpose of the Study:

  • To review common methods for quantifying plasma carnitine.
  • To discuss the advantages and disadvantages of these analytical approaches.

Main Methods:

  • Quantitative analysis of free and esterified carnitine.
  • Plasma and urine sample analysis.
  • Review of commonly employed quantification techniques.

Main Results:

  • Quantitative analysis of carnitine in plasma and urine is effective for assessing fatty acid transport and metabolism.
  • Various methods exist for plasma carnitine quantification, each with specific pros and cons.

Conclusions:

  • Plasma carnitine quantification is an effective diagnostic tool for carnitine-related disorders.
  • Understanding the strengths and weaknesses of different analytical methods is important for accurate clinical assessment.