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Related Experiment Video

Updated: Jan 23, 2026

Transport of Surface-modified Carbon Nanotubes through a Soil Column
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Nanoparticle-Modified Apoferritin Nanotransfer for Targeted Cytostatic Transport.

M Čížek, M Gargulák, K Sehnal

    Klinicka Onkologie : Casopis Ceske a Slovenske Onkologicke Spolecnosti
    |June 21, 2019
    PubMed
    Summary

    Apoferritin (APO) was engineered into a nanotransporter for doxorubicin (DOX) delivery, enhanced with gold and silver nanoparticles. This novel nanocomplex targets tumors via TfR binding and pH-responsive drug release for improved cancer therapy.

    Keywords:
    apoferritin nanotransportergold nanoparticlesmalignant tumorsnanomedicineprostate tumorssilver nanoparticlestargeted therapytransferin receptors

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    Area of Science:

    • Biomedical Engineering
    • Nanotechnology
    • Drug Delivery Systems

    Background:

    • Ferritin, an iron reservoir protein, is upregulated in malignancies.
    • Tumor cells, particularly prostate carcinoma, exhibit high transferrin receptor (TfR) expression.
    • Apoferritin (APO) serves as a nanotransporter for encapsulating therapeutic agents.

    Purpose of the Study:

    • To encapsulate doxorubicin (DOX) into APO.
    • To modify APO/DOX with gold nanoparticles (AuNPs) and green-synthesized silver nanoparticles (AgNPsGS).
    • To enhance therapeutic efficacy against tumors via targeted delivery and controlled release.

    Main Methods:

    • Characterization of APO and APO/DOX using SDS-PAGE and fluorescence spectroscopy.
    • Electrochemical measurements and pH-dependent stability studies of APO.
    • Green synthesis of AgNPsGS from clover and subsequent complexation with DOX and APO.
    • Coating APO/AgNPsGS-DOX with AuNPs for enhanced stability and drug release.

    Main Results:

    • APO exhibited pH-dependent structural changes, forming subunits in acidic and globular forms in alkaline environments.
    • A ~10% change in APO/DOX mobility was observed.
    • DOX release increased by up to 50% under specific conditions.
    • Stable APO/AgNPsGS-DOX/AuNPs nanocomplexes were formed, enabling pH and potentially photothermal-triggered DOX release.

    Conclusions:

    • APO nanocomplexes modified with AuNPs and AgNPsGS demonstrate potential for enhanced tumor targeting and drug delivery.
    • TfR binding and increased tumor cell permeability contribute to targeted delivery.
    • pH-induced drug release and potential photothermal activation offer promising therapeutic strategies.