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Updated: Jan 23, 2026

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
Published on: February 26, 2018
Activities of Biapenem against Mycobacterium tuberculosis in Macrophages and Mice
Zhen Yong Guo1, Wei Jie Zhao1, Mei Qin Zheng1
1Beijing Chest Hospital affiliated with the Capital Medical University/Beijing Tuberculosis and Thoracic Tumor Institute, Beijing 100049, China.
Objective:
To assess the activities of biapenem against multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis.
Methods:
Biapenem/clavulanate (BP/CL) was evaluated for in vitro activity against Mycobacterium tuberculosis (Mtb) multidrug-resistant (MDR) isolates, extensively drug-resistant (XDR) isolates, and the H37RV strain. BP/CL activity against the H37Rv strain was assessed in liquid cultures, in macrophages, and in mice..
Results:
BP/CL exhibited activity against MDR and XDR Mtb isolates in liquid cultures. BP/CL treatment significantly reduced the number of colony forming units (CFU) of Mtb within macrophages compared with control untreated infected macrophages. Notably, BP/CL synergized in pairwise combinations with protionamide, aminosalicylate, and capreomycin to achieve a fractional inhibitory concentration for each pairing of 0.375 in vitro. In a mouse tuberculosis infection model, the efficacy of a cocktail of levofloxacin + pyrazinamide + protionamide + aminosalicylate against Mtb increased when the cocktail was combined with BP/CL, achieving efficacy similar to that of the positive control treatment (isoniazid + rifampin + pyrazinamide) after 2 months of treatment.
Conclusion:
BP/CL may provide a new option to clinically treat MDR tuberculosis.
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