Label-Free Flow Multiplex Biosensing via Photonic Crystal Surface Mode Detection

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Oncology

Background:

• Circulating cancer markers are crucial for tumor diagnosis, treatment, and monitoring.
• Early cancer detection necessitates sensitive and rapid analytical methods.
• Existing methods may lack the speed or sensitivity required for timely diagnosis.

Purpose of the Study:

• To develop and validate a label-free, high-precision biosensing technique for simultaneous cancer marker detection.
• To assess the efficacy of the photonic crystal surface mode (PC SM) detection for multiple cancer biomarkers.
• To enable real-time, multiplexed detection of cancer antigens in a single experiment.

Main Methods:

• Utilized photonic crystal surface mode (PC SM) detection, a label-free biosensing technique.
• Employed real-time flow detection to monitor specific interactions between cancer antigens and capture antibodies.
• Developed a multichannel recording capability on a single chip for multiplexed analysis.

Main Results:

• Successfully detected ovarian cancer marker cancer antigen 125 and breast cancer markers human epidermal growth factor receptor 2 and cancer antigen 15-3 simultaneously.
• Demonstrated high specificity and sensitivity for the detection of all three targeted cancer biomarkers.
• Validated the PC SM technique's ability for real-time, multiplexed cancer marker analysis.

Conclusions:

• The developed PC SM biosensing approach provides a highly specific and sensitive platform for simultaneous cancer marker detection.
• This technique facilitates early cancer diagnosis and disease monitoring through rapid, multiplexed biomarker analysis.
• The multichannel capability offers significant advantages for clinical diagnostics and personalized medicine.