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Updated: Jan 23, 2026

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
Published on: March 31, 2017
Transcriptome sequence resource for the cucurbit powdery mildew pathogen Podosphaera xanthii
Rita Milvia De Miccolis Angelini1, Stefania Pollastro2, Palma Rosa Rotondo1
1Department of Soil, Plant and Food Sciences, University of Bari, Bari, Italy.
Researchers sequenced the transcriptome of Podosphaera xanthii, the fungus causing cucurbit powdery mildew. This study provides new genetic insights into the pathogen and its interactions with plants.
Area of Science:
- Mycology
- Plant Pathology
- Genomics
Background:
- Podosphaera xanthii is a significant pathogen causing cucurbit powdery mildew, particularly in Southern Italy.
- Understanding its genetic makeup is crucial for developing effective disease management strategies.
Purpose of the Study:
- To perform a de novo transcriptome assembly of Podosphaera xanthii using Illumina sequencing.
- To identify genes related to sexual reproduction, transposable elements, and potential mycoviruses in P. xanthii.
Main Methods:
- Illumina sequencing of mRNA from two P. xanthii isolates (MAT1-1 and MAT1-2) and their sexual cross.
- De novo Trinity-based assembly of the fungal transcriptome.
- Functional annotation of assembled transcripts using BLASTx.
Main Results:
- Over 60 million high-quality reads were obtained and assembled into 71,095 contigs.
- More than 55% of transcripts (40,221 contigs) showed significant BLASTx hits.
- Identified sequences related to sexual compatibility, reproduction, transposable elements, and putative mycoviruses.
Conclusions:
- The generated transcriptome data offer valuable resources for studying P. xanthii.
- These findings will enhance understanding of the pathogen's genetic variation and its interactions with host plants and the microbiome.
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