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Updated: Jan 23, 2026

The Sleep Nullifying Apparatus: A Highly Efficient Method of Sleep Depriving Drosophila
Published on: December 14, 2020
Abnormal hippocampal miR-1b expression is ameliorated by regular treadmill exercise in the sleep-deprived female rats
Lily Mohammadipoor-Ghasemabad1,2, Mohammad Hossein Sangtarash1, Saeed Esmaeili-Mahani3
1Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran.
Objectives:
The protective effect of regular running on sleep deprivation (SD)-induced cognitive impairment has been revealed. In this study, we focused on the effects of regular exercise, sleep deprivation and both of them together on the microRNA-1b (miR-1b) expression and their relation to the behavioral parameters and brain-derived neurotrophic factor (BDNF) expression.
Materials And Methods:
We used ovariectomized (OVX) female rats. The exercise program was mild-moderate treadmill training for 4 weeks. 72 hr SD was achieved using the multiple platform method and the spatial learning and memory parameters have been evaluated by the Morris water maze (MWM) test. The levels of studied genes were quantified by real-time PCR.
Results:
SD down-regulated pri-miR-1b, miR-1b (P˂0.05), and BDNF mRNA (P˂0.01) in the hippocampus. Furthermore, female rats under exercise conditions showed significant up-regulation of the miR-1b and BDNF mRNA (P˂0.001). In addition, miR-1b positively correlated with cognitive function (P˂0.05) and BDNF mRNA (P˂0.01).
Conclusion:
Our data demonstrated that regular treadmill exercise could reverse the down-regulation of hippocampal miR-1b, which has a probable role in the SD-induced cognitive impairment.
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