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Expression Profiling and Clinical Significance of Plasma MicroRNAs in Diabetic Nephropathy

Jianqin Wang1, Gouqin Wang1, Yaojun Liang1

  • 1Department of Nephropathy, Lanzhou University Second Hospital, No. 82 Cuiyingmen Lanzhou, Gansu province, China.

Journal of Diabetes Research
|June 21, 2019
PubMed
Abstract

Insights

Specific microRNAs (miRNAs) in plasma show altered expression in diabetic nephropathy (DN). miR-150-5p and miR-155-5p may serve as novel biomarkers for early DN detection and progression.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are stable biomarkers found in body fluids.
  • Investigating plasma miRNA profiles can aid in understanding diseases like diabetic nephropathy (DN).

Purpose of the Study:

  • To profile plasma miRNAs in diabetic nephropathy (DN) patients.
  • To determine the clinical significance of plasma miRNAs in DN pathogenesis and progression.

Main Methods:

  • Plasma samples from 66 DN patients, 36 diabetic patients without albuminuria, and 40 healthy controls were analyzed.
  • miRNA expression profiling was performed using miRNA low-density array chips and validated by quantitative real-time polymerase chain reaction.
  • Correlations between differential miRNA expression and clinicopathological parameters were assessed.

Main Results:

  • Five miRNAs (miR-150-5p, miR-155-5p, miR-30e, miR-320e, miR-3196) were differentially expressed across study groups (P < 0.05).
  • miR-150-5p and miR-155-5p levels were significantly higher in macroalbuminuria patients compared to microalbuminuria patients.
  • Plasma miR-150-5p and miR-155-5p levels correlated negatively with albuminuria excretion rate and positively with estimated glomerular filtration rate.

Conclusions:

  • miR-150-5p, miR-155-5p, miR-30e, miR-320e, and miR-3196 show potential as diagnostic biomarkers for early DN.
  • miR-150-5p and miR-155-5p may play roles in DN pathogenesis and progression.
  • Further research is needed to validate these findings and elucidate molecular mechanisms.

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