Improved Stability and Enhanced Anti-Tumor Activity of Hyaluronic Acid Modified ES2-AF Nanoparticle-Like Conjugate
Abstract:
The growth, migration and spread of malignant tumors requires a large number of new blood vessels to supply nutrients. In our previous study, it was found that the hyaluronic acid (HA) modified novel antiangiogenic peptide ES2-AF has better solubility, longer half-life, stronger targeting than non-modified ES2-AF, and it significantly inhibited the formation of new blood vessels. In the current work, we studied the stability of ES2-AF after HA modification, including heat stability, longterm stability, and pH stability. After treatment with HA-ES2-AF, cell apoptosis and the cell cycle were analyzed using flow cytometry. The inhibitory effect of HA-ES2-AF on tumors was investigated in vivo in HepG2 tumor-bearing nude mice. All the above results showed that HA-ES2-AF significantly improved stability, effectively induced apoptosis of endothelial cells, caused G1 phase arrest of endothelial cells, and decreased the percentage of cells in G2 and S phases. In vivo, HA-ES2-AF exhibited an inhibitory effect on tumors growth. In addition, the results provide a reliable basis for studying the inhibition of neovascularization and anti-tumor drugs in the future.
Related Concept Videos
Stability of Conjugated Dienes
A comparison of the enthalpies of hydrogenation of dienes reveals that conjugated dienes release less heat on hydrogenation, rendering them more stable than their nonconjugated analogs.
Relative Strengths of Conjugate Acid-Base Pairs
Bioavailability Enhancement: Drug Stability Enhancement and GI Retention
Conjugated Proteins
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
RNA Stability
Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...


