EEG-correlated fMRI of human alpha (de-)synchronization

We investigated blood oxygenation level-dependent (BOLD) brain activity changes in wakefulness and light sleep and in relation to those associated with the posterior alpha rhythm, the most prominent feature of the clinical EEG. Studies have reported different sets of brain regions changing their oxygen consumption with waxing and waning alpha oscillations. Here, we hypothesize that these dissimilar activity patterns reflect different wakefulness-dependent brain states.

We recorded BOLD signal changes and electroencephalography (EEG) simultaneously in 149 subjects at rest. Based on American Academy of Sleep Medicine criteria, we selected subjects exhibiting wakefulness or light sleep (N1). We identified brain regions in which BOLD signal changes correlated with (i) clinical sleep stages, (ii) alpha band power and (iii) a multispectral EEG index, respectively.

During light sleep, we found increased BOLD activity in parieto-occipital regions. In wakefulness compared to light sleep, we revealed BOLD signal increases in the thalamus. The multispectral EEG-index revealed hippocampal activity changes in light sleep not reported before.

Changes in alpha oscillations reflect different brain states associated with different levels of wakefulness and thalamic activity. We can link the previously described parieto-occipital pattern to drowsiness. Additionally, in that stage, we identify hippocampal activity fluctuations.

Thalamic activity varies with early changes of wakefulness, which is important to consider in resting state experiments. The EEG-indexed activation of the hippocampus during light sleep suggests that memory encoding might already take place during this early stage of sleep.