Related Experiment Video

Updated: Jan 23, 2026

Tear-Derived Exosomal miR-15a as New Diagnostic Tool for Diabetic Retinopathy
07:45

Tear-Derived Exosomal miR-15a as New Diagnostic Tool for Diabetic Retinopathy

Published on: December 30, 2025

314

miR-15a/-16 Inhibit Angiogenesis by Targeting the Tie2 Coding Sequence: Therapeutic Potential of a miR-15a/16 Decoy

Marie Besnier1, Saran Shantikumar1, Maryam Anwar2

  • 1Bristol Heart Institute, University of Bristol, Bristol, UK.

Molecular Therapy. Nucleic Acids
|June 21, 2019
PubMed

Insights

MicroRNA-15a (miR-15a) and miR-16 inhibit blood vessel formation after ischemia. A novel decoy system targeting these microRNAs improved blood flow and tissue recovery in a mouse limb ischemia model.

Area of Science:

  • Molecular Biology
  • Cardiovascular Biology
  • Gene Regulation

Background:

  • MicroRNA-15a (miR-15a) and miR-16 are known to inhibit post-ischemic angiogenesis.
  • MicroRNA (miRNA) binding to messenger RNA (mRNA) coding sequences (CDSs) is a key gene expression regulatory mechanism.

Purpose of the Study:

  • Identify novel mediators of miR-15a/16 anti-angiogenic effects.
  • Develop an adenovirus (Ad)-based system to sense and inhibit miR-15a/16 activity.
  • Evaluate the therapeutic potential of this decoy system in a mouse limb ischemia (LI) model.

Main Methods:

  • Utilized bioinformatic tools and luciferase assays to identify miR-15a/16 targets.
  • Overexpressed and inhibited miR-15a/16 in cultured endothelial cells (ECs).
  • Delivered an Ad-based miR-15a/16 decoy system (Ad.Luc-Decoy-15a/16) locally in a mouse LI model and assessed outcomes using bioluminescent imaging.

Main Results:

  • miR-15a and miR-16 expression increased in ECs during limb ischemia and serum starvation.
  • miR-15a/16 directly bind to the Tie2 coding sequence, reducing Tie2 protein levels.
  • Inhibition of miR-15a/16 enhanced angiogenesis in a Tie2-dependent manner.
  • Local delivery of Ad.Luc-Decoy-15a/16 restored Tie2 levels, improved angiogenesis, perfusion, and reduced necrosis in the mouse LI model.
  • Bioluminescent imaging confirmed the Ad.Luc-Decoy-15a/16 system's responsiveness to elevated miR-15a/16.

Conclusions:

  • Novel mechanistic insight into miR-15a/16-mediated inhibition of angiogenesis via Tie2.
  • Demonstrated the therapeutic efficacy of targeting miR-15a/16 using a decoy system for limb ischemia.
  • Established a responsive bioluminescent reporter system for monitoring miR-15a/16 activity in vivo.
Keywords:
IVISTie2angiogenesisangiopoietinsischemiamiR decoymiR-15amiR-16amicroRNAsmouse ischemia model

More Related Videos

Assessing Therapeutic Angiogenesis in a Murine Model of Hindlimb Ischemia07:48

Assessing Therapeutic Angiogenesis in a Murine Model of Hindlimb Ischemia

Published on: June 8, 2019

9.2K
Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells09:58

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Published on: May 2, 2017

8.0K

Related Concept Videos

Cattell's 16 Personality Factors01:24

Cattell's 16 Personality Factors

Raymond Cattell's trait theory offers a structured framework for understanding personality by distinguishing between two critical traits: surface and source traits. Surface traits are observable patterns of behavior, such as indecisiveness, anxiety, and irrational fears. These traits are less stable, varying across situations and over time. This means that they are less helpful in understanding the deeper aspects of an individual's personality.
In contrast, source traits are the...
2.4K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.8K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.6K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.6K
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
56.9K
Nursing Code of Ethics01:29

Nursing Code of Ethics

The Nursing Code of Ethics sets the ethical benchmark for the profession, and guides nurses in ethical analysis and decision making at the societal, organizational, and clinical levels. The code encompasses showing compassion and respect for the patient, their families, and communities in all circumstances while committing to providing patient-centered care. In addition, the code states that nurses must advocate for the patient by defending a cause or recommendation to protect their rights,...
4.3K