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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Related Experiment Video

Updated: Jan 1, 2026

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Published on: August 26, 2025

[Progress in gene knockout mice].

Chaoxuan Wang¹, Hang Sun¹

¹Institute of Viral Hepatitis, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology

|June 22, 2019

Summary

This summary is machine-generated.

Gene knockout mice are vital for studying gene function and disease. This review highlights new techniques like CRISPR/Cas9 and TALEN, building on established methods for creating these essential research models.

Keywords:

embryonic stem cells gene editing gene knockout technology gene targeting homologous recombination

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Last Updated: Jan 1, 2026

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Area of Science:

Genetics and Genomics
Animal Models
Molecular Biology

Background:

Gene knockout mice are indispensable tools for understanding gene function and modeling human diseases.
Traditional methods like gene targeting and gene trap have been foundational in generating these models.
Advances in genetic engineering have led to the development of more efficient and precise knockout techniques.

Purpose of the Study:

To provide an overview of established and novel techniques for generating gene knockout mice.
To focus on recent advancements in gene knockout methodologies.
To highlight the significance of these techniques in biological research and disease treatment.

Main Methods:

Review of established gene targeting and gene trap strategies.
Discussion of the Cre/loxP recombination system.
Exploration of newer technologies: CRISPR/Cas9, ZFN, and TALEN.

Main Results:

Gene targeting utilizes homologous recombination to replace endogenous genes.
Gene trap methods include promoter trap and polyA trap.
Newer systems like CRISPR/Cas9 offer enhanced precision and efficiency in gene editing for knockout mouse generation.

Conclusions:

Gene knockout mouse technology has evolved significantly, offering powerful support for biological research.
Emerging techniques like CRISPR/Cas9, ZFN, and TALEN represent major advancements in creating gene knockout models.
These refined methods accelerate the study of gene function and the development of therapeutic strategies for human diseases.