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LncRNA GASL1 is downregulated in chronic heart failure and regulates cardiomyocyte apoptosis

Haihong Deng1, Wenbo Ouyang1, Li Zhang2

  • 11Department of Anesthesiology, The First People's Hospital of Zhaoqing, Zhaoqing City, Guangdong Province 526000 People's Republic of China.

Cellular & Molecular Biology Letters
|June 22, 2019
PubMed

Insights

Long non-coding RNA GASL1 is downregulated in chronic heart failure (CHF). Overexpressing GASL1 may improve CHF by reducing heart cell apoptosis via TGF-β1 inactivation.

Area of Science:

  • Cardiology
  • Molecular Biology
  • Genetics

Background:

  • Transforming growth factor-beta 1 (TGF-β1) is implicated in chronic heart failure (CHF).
  • Long non-coding RNA GASL1 is known to inactivate TGF-β1 in cancer biology.

Purpose of the Study:

  • To investigate the role of lncRNA GASL1 in chronic heart failure.
  • To explore the relationship between GASL1, TGF-β1, and cardiomyocyte apoptosis in CHF.

Main Methods:

  • Plasma samples from CHF patients and controls were analyzed using ELISA and RT-qPCR.
  • Cardiomyocyte experiments involved vectors, transient transfections, and western blot.
  • Cellular apoptosis was assessed under H2O2 treatment.

Main Results:

  • CHF patients exhibited higher plasma TGF-β1 and lower GASL1 levels.
  • GASL1 and TGF-β1 were inversely correlated in CHF patients.
  • GASL1 overexpression downregulated TGF-β1 in cardiomyocytes and reduced apoptosis, while TGF-β1 overexpression increased apoptosis.

Conclusions:

  • GASL1 is downregulated in CHF.
  • GASL1 overexpression demonstrates potential therapeutic benefits for CHF by inhibiting cardiomyocyte apoptosis through TGF-β1 inactivation.
Abstract

Keywords:
ApoptosisChronic heart failureTGF-β1lncRNA GASL1

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