Related Experiment Video
Updated: Aug 10, 2026

Optical Clearing and Imaging of Immunolabeled Kidney Tissue
Published on: July 22, 2019
Immuno-light and immuno-electron microscopic examination of freeze-dried kidney tissue
1Department of Pathology I, Linköping University, Sweden.
Abstract:
Correlative morphology of kidney tissue can be accomplished by a combination of immunofluorescent staining and light microscopy using a pre-embedding technique. Further development of this method has been elaborated by freeze-drying and chopping of the tissue, which then is incubated in biotinylated immunoglobulins. Fluorochromes and colloidal gold can be coupled to the biotinylated immunoglobulins in a second step. This technique is an alternative to postembedding immunogold staining for immunoelectron microscopy, and permits a correlative immunohistochemical and detailed morphological study of kidney tissue.
Related Concept Videos
Immunogold Electron Microscopy
Cryo-electron Microscopy

