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Correlations of quantitative MRI metrics with myelin basic protein (MBP) staining in a murine model of demyelination

Lucas Soustelle1, Maria C Antal1, Julien Lamy1

  • 1Université de Strasbourg, CNRS, ICube, FMTS, Strasbourg, France.

NMR in Biomedicine
|June 22, 2019
PubMed

Insights

This study compared MRI techniques for myelin imaging in demyelination. Macromolecular proton fraction and Diff-UTE signal showed the strongest correlation with myelin basic protein histology in mice.

Area of Science:

  • Neuroimaging
  • Biophysics
  • Histology

Background:

  • Myelin imaging is crucial for detecting white matter diseases.
  • Quantitative MRI methods can detect myelin disruption.
  • Several MRI parameters show promise for myelin assessment.

Purpose of the Study:

  • To evaluate the sensitivity and correlation of various MRI parameters with myelin basic protein (MBP) histology.
  • To compare quantitative magnetization transfer imaging (qMTI), diffusion tensor imaging (DTI), myelin water imaging (MWI), and Diff-UTE in a demyelination model.

Main Methods:

  • A murine model of demyelination was studied at 7T MRI.
  • Quantitative MRI parameters including qMTI (bound pool fraction), DTI (radial diffusivity), MWI (myelin water fraction), and Diff-UTE (rSPF) were acquired.
  • MRI results were correlated with immunohistological assessment of MBP.

Main Results:

  • All tested MRI metrics demonstrated sensitivity to demyelination.
  • Strong correlations were found between MBP histology and macromolecular proton fraction (ρ>0.87) and Diff-UTE signal (ρ>0.76) in the corpus callosum.
  • Moderate correlations were observed with radial diffusivity and myelin water fraction (|ρ|<0.70).

Conclusions:

  • Macromolecular proton fraction and Diff-UTE signal are highly sensitive MRI metrics for myelin assessment in demyelination.
  • These advanced MRI techniques show significant potential for clinical translation in white matter disease monitoring.
Keywords:
UTE imagingdemyelinationdiffusion tensor imaginghistologymyelin basic proteinmyelin water imagingquantitative magnetization transfer

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