Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...

Colors and Magnetism

Colors and Magnetism

Color in Coordination Complexes
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human...

Health Information Technology and Healthcare Information System

Health Information Technology and Healthcare Information System

Health Information Technology (HIT)
Health Information Technology, commonly called HIT, integrates advanced information systems and technology in healthcare settings. Its primary functions include:

Magnetism

Magnetism

Magnets are commonly found in everyday objects, such as toys, hangers, elevators, doorbells, and computer devices. Experimentation on these magnets shows that all magnets have two poles: one is labeled north (N) and the other south (S). Magnetic poles repel if they are alike and attract if unlike. Moreover, both poles of a magnet attract unmagnetized pieces of iron.
An individual magnetic pole cannot be isolated. No matter how small, every piece of a magnet contains a north pole and a south...

Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...

Magnetic Flux

Magnetic Flux

The magnetic flux measures the number of magnetic field lines passing through a given surface area. The SI unit for magnetic flux is the weber (Wb). Magnetic flux is a scalar quantity. It depends on three factors: the strength of the magnetic field B, the area through which the field lines pass, and the relative orientation of the field with the surface area.
Suppose a surface is divided into elements of area dA. For each element, the component of the magnetic field that is normal to the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Computationally guided cross-linking overcomes interfacial mismatch in protein dimerization: Creating a long-acting cocaine esterase.

International journal of biological macromolecules·2026

Same author

A high-throughput fluorescent DNA cleavage assay for discovering bacterial DNA gyrase poisons.

Nucleic acids research·2026

Same author

Identification of Nsp15 inhibitors restoring interferon-dependent antiviral activity against SARS-CoV-2.

Antiviral research·2026

Same author

A high throughput assay to identify modulators of Death Receptor 3 (DR3).

SLAS discovery : advancing life sciences R & D·2026

Same author

Substrate specificity and in vivo efficacy of engineered cocaine esterases toward cocaine and its toxic metabolites.

Chemico-biological interactions·2026

Same author

A SARS-CoV-2 entry inhibitor trimerizes to lock the spike protein in a closed conformation.

bioRxiv : the preprint server for biology·2026

Search research articles

Related Experiment Video

Updated: Jan 23, 2026

Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening

Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening

Published on: September 5, 2018

Automating a Magnetic 3D Spheroid Model Technology for High-Throughput Screening.

Pierre Baillargeon¹, Justin Shumate¹, Shurong Hou^1,2

¹1 The Scripps Research Molecular Screening Center, Department of Molecular Medicine, Scripps Florida, Jupiter, FL, USA.

SLAS Technology

|June 22, 2019

Summary

Researchers developed a novel robotic system for high-throughput screening (HTS) using 3D cell cultures. This system enables rapid 3D structure formation and integrates 3D bioprinting with automated HTS platforms.

Keywords:

3D printing HTS magnetic bioprinting organoid spheroid

More Related Videos

A 3D Spheroid Model for Glioblastoma

A 3D Spheroid Model for Glioblastoma

Published on: April 9, 2020

An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion

An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion

Published on: March 31, 2022

Related Experiment Videos

Last Updated: Jan 23, 2026

Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening

Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening

Published on: September 5, 2018

A 3D Spheroid Model for Glioblastoma

A 3D Spheroid Model for Glioblastoma

Published on: April 9, 2020

An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion

An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion

Published on: March 31, 2022

Area of Science:

Biotechnology
Drug Discovery
3D Cell Culture

Background:

Three-dimensional (3D) cell-based assays are increasingly vital for early drug discovery.
Novel microplate treatments and spheroid culturing techniques enhance these assays.
Existing methods for 3D cell culture in high-throughput screening (HTS) can be limited in throughput.

Purpose of the Study:

To transition a manual 3D cell culture technique to a robotically enabled, high-throughput format.
To develop specialized hardware for automated robotic handling and precise microplate placement.
To create a first-in-class HTS-compatible 3D system integrating 3D bioprinting and automation.

Main Methods:

Utilized nanoparticle-labeled cells (NanoShuttle-PL) and magnetic drives for rapid 3D spheroid formation.
Developed custom ancillary devices using rapid prototyping and 3D printing for robotic integration.
Integrated a magnetic incubator shelf assembly into a fully automated HTS robotic platform.

Main Results:

Achieved orders of magnitude increase in throughput compared to manual methods.
Successfully developed and tested specialized hardware for robotic cell assay handling.
Created a novel, automated HTS-compatible system coupling 3D bioprinting with magnetic incubation.

Conclusions:

The developed system offers an affordable and physiologically relevant 3D cell-based assay for HTS.
Rapid prototyping and 3D printing enabled cost-effective and timely development of specialized hardware.
This work presents a significant advancement in automated 3D cell culture for drug discovery.