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Published on: December 27, 2024
Development, characterization and pre-clinical trials of an innovative wound healing dressing based on propolis
Franciane Marquele-Oliveira1, Hernane da Silva Barud2, Elina Cassia Torres3
1Laboratório de Pesquisa, Desenvolvimento e Inovação (P, D & I), Apis Flora Industrial e Comercial Ltda., Rua Triunfo 945, 14020-670 Ribeirão Preto, SP, Brazil.
Abstract:
The considerable role of pristine bacterial cellulose membranes (BC) as ideal dressings have been widely demonstrated to treat wounds and burns. Nevertheless, drawbacks regarding antimicrobial spectrum and frequent dressing replacement are still present. Based on this, the present work proposes an innovative dressing by incorporating a technological self-microemulsifying formulation (SMEF) encapsulating propolis (BC/PP). BC/PP was fully chemically and biologically characterized employing in vitro and in vivo models. Antimicrobial studies demonstrated BC/PP high efficiency against both gran-negative and gran-positive bacteria. Release studies evidenced propolis markers sustained release for up to 7 days. In vivo wound healing activity was assessed by wound healing rate, anti-inflammatory and tissue formation events and the results evidenced the pro-inflammatory activity of BC/PP, which could promote improved healing results. To conclude, BC/PP presented an outstanding antibacterial activity in vitro with weekly replacement and promotion of healing, offering, for the first time, a broad-spectrum biomembrane potential to treat infected wounds.
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