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Genes coding for LysM domains in the dermatophyte Trichophyton rubrum: A transcription analysis

Lúcia Lopes1, Tamires A Bitencourt1, Elza A S Lang1

  • 1Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.

Medical Mycology
|June 22, 2019
PubMed

Insights

Trichophyton rubrum, a fungus causing skin infections, has proteins with LysM domains that may be key to its virulence. Researchers studied these genes, finding some change activity when grown on keratin.

Area of Science:

  • Medical Mycology
  • Molecular Biology
  • Fungal Pathogenesis

Background:

  • Superficial mycoses caused by Trichophyton rubrum are increasing, particularly in immunocompromised individuals.
  • Understanding dermatophyte virulence mechanisms is crucial due to rising infection rates.
  • Genes encoding LysM domain proteins are abundant in dermatophytes, suggesting a role in virulence, but their function remains unclear.

Purpose of the Study:

  • To investigate the function of LysM domain-containing proteins in the human pathogen Trichophyton rubrum.
  • To analyze the transcriptional changes of these genes in response to different growth substrates, specifically keratin.
  • To contribute to understanding the role of LysM domains in dermatophyte virulence.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) was used to assess gene transcription.
  • Trichophyton rubrum was cultured on various substrates, including keratin.
  • In silico analyses were performed to predict protein features and functions.

Main Results:

  • Several genes encoding LysM domain proteins exhibited altered transcription levels when T. rubrum was grown on keratin.
  • In silico analysis indicated that some of these proteins are plasma membrane-anchored and possess chitinase II and signal peptide domains.
  • This study provides a detailed examination of LysM domain-containing genes in T. rubrum.

Conclusions:

  • LysM domain-containing proteins are potential virulence factors in Trichophyton rubrum.
  • Keratin, a major component of skin, influences the expression of these genes.
  • Further research into these proteins could reveal new therapeutic targets for dermatophytosis.
Keywords:
Trichophyton rubrumLysM domaindermatophytefungal virulencegene expression

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