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Updated: Aug 12, 2026
Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
Published on: August 9, 2011
Effect of Prompt Inoculator on results of Sceptor system minimal inhibitory concentration determinations for Listeria
1Clinical Microbiology Laboratories, University of Kentucky Medical Center, Lexington.
Abstract:
The minimal inhibitory concentrations (MICs) of eight antimicrobial agents were determined for each of 36 isolates of Listeria monocytogenes with the Sceptor system. Two different inoculation procedures, the standard Sceptor log-phase broth (LB) culture and the Prompt Inoculator (PI) (Bauer-Kirby type), were used. The PI MIC/LB MIC ratio (PI/LB ratio) was determined for each antimicrobial agent for each of the isolates. Of the 217 on-scale PI/LB ratio results, all were within the expected range of 0.5 to 2.0. The PI was found to be a convenient, rapid, and suitable method of preparing an inoculum of L monocytogenes for use with the Sceptor system and should function equally well when testing L monocytogenes isolates with other commercially available MIC systems.
