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Updated: Jan 23, 2026
10:19
Analysis and Imaging of Osteocytes
Published on: November 29, 2024
1.3K
Investigating Osteocytic Perilacunar/Canalicular Remodeling
Cristal S Yee1, Charles A Schurman1,2, Carter R White1
1Department of Orthopaedic Surgery, University of California, 513 Parnassus Avenue, San Francisco, CA, 94143, USA.
Current Osteoporosis Reports
|June 23, 2019
Summary
Perilacunar/canalicular remodeling (PLR) is vital for skeletal homeostasis. This review examines methods for analyzing PLR, emphasizing standardized outcomes for better understanding osteocyte function in skeletal health and disease.
Area of Science:
- Skeletal Biology
- Cellular Biology
- Biomineralization
Background:
- Osteocytes dynamically remodel the pericellular matrix via perilacunar/canalicular remodeling (PLR).
- The osteocyte lacuna-canalicular network (LCN) has a large surface area, making PLR crucial for skeletal homeostasis.
- Understanding PLR mechanisms is key to skeletal health and disease research.
Purpose of the Study:
- To review the motivations and critical considerations for analyzing PLR.
- To examine PLR analysis in both in vitro and in vivo systems.
- To highlight the importance of PLR in maintaining skeletal integrity.
Main Methods:
- Discusses current outcomes used to assess PLR, including enzyme expression and LCN morphology.
- Reviews the application of these outcomes in studying PLR induction and suppression.
- Emphasizes the need for integrated analysis of PLR data.
Main Results:
- Morphological approaches alone are insufficient to understand PLR regulation.
- Standardized PLR outcomes are necessary for routine skeletal phenotyping.
- Improved molecular and cellular outcomes are needed for comprehensive PLR analysis.
Conclusions:
- Integrated analysis of PLR has the potential to reveal novel osteocyte functions in skeletal health and disease.
- Standardized and improved outcomes will advance the study of PLR.
- Further research into PLR mechanisms is critical for skeletal health.
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