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Updated: Jan 23, 2026

Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
Scanning electrochemical microscopy in the development of enzymatic sensors and immunosensors
Inga Morkvenaite-Vilkonciene1, Almira Ramanaviciene2, Aura Kisieliute3
1NanoTechnas - Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko 24, LT-03225, Vilnius, Lithuania; Department of Mechatronics and Robotics, Vilnius Gediminas Technical University, J. Basanavičiaus 28, 03224, Vilnius, Lithuania.
Abstract:
Scanning electrochemical microscopy (SECM) is very useful, non-invasive tool for the analysis of surfaces pre-modified with biomolecules or by whole cells. This review focuses on the application of SECM technique for the analysis of surfaces pre-modified with enzymes (horseradish peroxidase, alkaline phosphatase and glucose oxidase) or labelled with antibody-enzyme conjugates. The working principles and operating modes of SECM are outlined. The applicability of feedback, generation-collection and redox competition modes of SECM on surfaces modified by enzymes or labelled with antibody-enzyme conjugates is discussed. SECM is important in the development of miniaturized bioanalytical systems with enzymes, since it can provide information about the local enzyme activity. Technical challenges and advantages of SECM, experimental parameters, used enzymes and redox mediators, immunoassay formats and analytical parameters of enzymatic SECM sensors and immunosensors are reviewed.
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