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Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells
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Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells

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HIF-1α induced long noncoding RNA FOXD2-AS1 promotes the osteosarcoma through repressing p21

Zhipeng Ren1, Yongcheng Hu2, Guishi Li3

  • 1Department of Orthopaedics, Tianjin Hospital, Tianjin, 300211, China; Graduate School, Tianjin Medical University, Tianjin, 300070, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|June 23, 2019
PubMed

Insights

FOXD2-AS1 is highly expressed in osteosarcoma (OS) and promotes tumor growth by inhibiting p21. This long noncoding RNA, regulated by HIF-1α, represents a potential therapeutic target for OS patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) play crucial roles in osteosarcoma (OS) development.
  • The specific regulatory function of FOXD2-AS1 in OS remains unclear.

Purpose of the Study:

  • To investigate the role and mechanism of FOXD2-AS1 in osteosarcoma.
  • To explore FOXD2-AS1 as a potential therapeutic target for OS.

Main Methods:

  • Quantitative real-time PCR to measure FOXD2-AS1 expression in OS tissues and cell lines.
  • Analysis of the correlation between FOXD2-AS1 expression and clinical prognosis.
  • Investigation of HIF-1α binding to the FOXD2-AS1 promoter.
  • In vitro and in vivo experiments to assess the functional impact of FOXD2-AS1 knockdown.
  • Western blot and co-immunoprecipitation assays to elucidate the interaction between FOXD2-AS1, EZH2, and p21.

Main Results:

  • FOXD2-AS1 expression was significantly upregulated in OS tissues and cell lines, correlating with poor prognosis.
  • Transcription factor HIF-1α activates FOXD2-AS1 transcription in OS cells.
  • Knockdown of FOXD2-AS1 suppressed OS cell proliferation, invasion, and tumor growth in vitro and in vivo.
  • FOXD2-AS1 interacts with EZH2 to epigenetically silence p21 expression, inhibiting tumor suppressor activity.

Conclusions:

  • FOXD2-AS1 acts as an oncogene in osteosarcoma tumorigenesis, induced by HIF-1α.
  • FOXD2-AS1 promotes OS progression by interacting with EZH2 to repress p21.
  • Targeting FOXD2-AS1 may offer a novel therapeutic strategy for osteosarcoma.
Keywords:
EZH2FOXD2-AS1HIF-1αOsteosarcomap21

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