Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Behavior?00:54

What is Behavior?

10.2K
Behaviors are actions that an organism engages in—they can be related to finding food, reproducing, defending against threats, and many other possible actions. Behaviors include activities related to the environment around the animal—such as migration—as well as social interactions within a species or population. Many behaviors involve motor output—that is, muscle movements—while others involve less visible actions, such as learning.
10.2K
Structures of Solids02:22

Structures of Solids

17.5K
Solids in which the atoms, ions, or molecules are arranged in a definite repeating pattern are known as crystalline solids. Metals and ionic compounds typically form ordered, crystalline solids. A crystalline solid has a precise melting temperature because each atom or molecule of the same type is held in place with the same forces or energy. Amorphous solids or non-crystalline solids (or, sometimes, glasses) which lack an ordered internal structure and are randomly arranged. Substances that...
17.5K
Structural Isomerism02:34

Structural Isomerism

21.5K
Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
21.5K
Structure of Lipids03:38

Structure of Lipids

98.4K
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
98.4K
Viral Structure00:56

Viral Structure

74.1K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
74.1K
Antibody Structure01:10

Antibody Structure

65.4K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serological diagnosis of canine leptospirosis using a chimeric protein based on bacterial surface antigens.

Microbial pathogenesis·2026
Same author

Biochemical and Structural Characterization of Two-domain Glycoside Hydrolase PgaB from <i>Serratia marcescens</i> and Its Application for <i>S. aureus</i> Biofilm Degradation.

ACS infectious diseases·2026
Same author

NEK6 Knockout Causes Defects in Mitochondrial Morphology and Respiration.

Cells·2026
Same author

Oncogenic Role of SRPK2 in Different Types of Cancer: A Systematic Review.

Journal of cellular and molecular medicine·2026
Same author

Mapping Potential Risks for the Transmission of Spotted Fever Rickettsiosis after Environmental Changes in an Atlantic Forest Region in Brazil.

ACS omega·2026
Same author

Recombinant LPG3 Protein From Leishmania chagasi as an Antigen for the Diagnosis of Canine Visceral Leishmaniasis.

Tropical medicine & international health : TM & IH·2026

Related Experiment Video

Updated: Jan 23, 2026

Advanced Self-Healing Asphalt Reinforced by Graphene Structures: An Atomistic Insight
08:03

Advanced Self-Healing Asphalt Reinforced by Graphene Structures: An Atomistic Insight

Published on: May 31, 2022

5.6K

Insights into the full-length SRPK2 structure and its hydrodynamic behavior.

Éverton de Almeida Alves Barbosa1, Thiago Vargas Seraphim2, César Augusto Gandin3

  • 1Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, Brazil.

International Journal of Biological Macromolecules
|June 24, 2019
PubMed
Summary

Serine/arginine-rich protein kinase 2 (SRPK2) is a cancer target. This study characterized full-length SRPK2 in solution, revealing unique flexible regions and an elongated shape, distinct from truncated versions.

More Related Videos

Protocol for Relative Hydrodynamic Assessment of Tri-leaflet Polymer Valves
11:12

Protocol for Relative Hydrodynamic Assessment of Tri-leaflet Polymer Valves

Published on: October 17, 2013

14.2K
A Microfluidic-based Hydrodynamic Trap for Single Particles
10:13

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 21, 2011

17.2K

Related Experiment Videos

Last Updated: Jan 23, 2026

Advanced Self-Healing Asphalt Reinforced by Graphene Structures: An Atomistic Insight
08:03

Advanced Self-Healing Asphalt Reinforced by Graphene Structures: An Atomistic Insight

Published on: May 31, 2022

5.6K
Protocol for Relative Hydrodynamic Assessment of Tri-leaflet Polymer Valves
11:12

Protocol for Relative Hydrodynamic Assessment of Tri-leaflet Polymer Valves

Published on: October 17, 2013

14.2K
A Microfluidic-based Hydrodynamic Trap for Single Particles
10:13

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 21, 2011

17.2K

Area of Science:

  • Biochemistry
  • Structural Biology
  • Molecular Biology

Background:

  • Serine/arginine-rich protein kinase 2 (SRPK2) is upregulated in various cancers, contributing to cell migration, growth, and apoptosis.
  • SRPK2 is a promising drug discovery target, but high-resolution structures are limited to truncated forms.
  • Intrinsically unstructured regions in SRPK2 hinder structural characterization of full-length protein.

Purpose of the Study:

  • To characterize the solution structure of full-length recombinant SRPK2.
  • To compare the structural properties of full-length SRPK2 with a truncated version.
  • To define unique structural features of SRPK2 within the SRPK family.

Main Methods:

  • Small-Angle X-ray Scattering (SAXS) for low-resolution structural data.
  • Analytical Size Exclusion Chromatography (SEC) for hydrodynamic behavior.
  • Sedimentation Velocity Analytical Ultracentrifugation (SV-AUC) for molecular weight and shape determination.

Main Results:

  • Full-length SRPK2 exists mainly as dimers in solution, unlike the truncated version which showed dimerization propensity at higher concentrations.
  • SRPK2 exhibits a monomer-dimer equilibrium in solution.
  • Both monomeric and dimeric forms of SRPK2 possess an elongated shape, indicating conformational flexibility.
  • Flexible regions outside the bipartite kinase domain contribute to SRPK2's unique structural characteristics.

Conclusions:

  • Full-length SRPK2 displays distinct structural features compared to truncated variants, characterized by its elongated shape and conformational plasticity.
  • The identified flexible regions are unique to SRPK2 within the SRPK family.
  • Understanding SRPK2's solution structure provides insights for targeted drug development in cancer therapy.