Salidroside Reduces Inflammation and Brain Injury After Permanent Middle Cerebral Artery Occlusion in Rats by...

Area of Science:

• Neuroscience
• Pharmacology
• Biochemistry

Background:

• Salidroside, derived from Rhodiola rosea, shows neuroprotection in transient stroke models.
• Its efficacy in permanent stroke models requires further investigation.

Purpose of the Study:

• To evaluate salidroside's neuroprotective effects in a permanent middle cerebral artery occlusion (pMCAO) rat model.
• To elucidate the underlying molecular mechanisms of salidroside's action.

Main Methods:

• Rats underwent permanent MCAO and received daily intraperitoneal injections of salidroside.
• Cerebral infarct volume, neurological deficit scores, and specific protein expressions (NeuN, Nrf2, HO-1, NFκB p50, IL-6, TNFα, p-PKB/PKB, C3, Egr1) were assessed.
• Inhibitors of Nrf2 (brusatol) and PI3K (LY294002) were used to probe signaling pathways.

Main Results:

• 100 mg/kg/day salidroside significantly reduced infarct volume and neurological deficits by day 7 post-pMCAO.
• Salidroside increased NeuN, Nrf2, HO-1, and p-PKB/PKB, while decreasing NFκB p50, IL-6, and TNFα.
• The PI3K/PKB/Nrf2/NFκB pathway mediated these effects, independent of complement C3.

Conclusions:

• Salidroside demonstrates significant neuroprotective effects in a pMCAO rat model.
• The mechanism involves the PI3K/PKB/Nrf2/NFκB signaling pathway, reducing neuroinflammation and neural damage.
• Salidroside's therapeutic potential for stroke warrants further study.