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Culture of Preimplantation Rabbit Embryos.

Methods in molecular biology (Clifton, N.J.)·2019

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Historical Note: Lessons Learned from Developing the First Semi-Defined Medium for Culture of One-Cell Rabbit Embryos

Michael T Kane1

  • 1Department of Physiology, National University of Ireland Galway, Galway, Ireland. michael.kane@nuigalway.ie.

Methods in Molecular Biology (Clifton, N.J.)
|June 24, 2019
PubMed
Summary

This study details the historical development of semi-defined media for mammalian embryo culture, focusing on rabbit embryos. It highlights the challenges and discoveries that advanced in vitro fertilization techniques.

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Early mammalian embryo culture predominantly used rabbits with ill-defined media until the 1950s.
Keywords:
Amino acidsBlastocystCultureEmbryoHistoryHumanInositolMediumPreimplantationRabbit

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  • Mouse embryo culture advanced rapidly with defined media after 1949.
  • A resurgence in rabbit embryo culture in the 1960s revealed distinct nutritional needs compared to mouse embryos.