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Updated: Jan 23, 2026
06:53
Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
Published on: July 10, 2021
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Small Ruminants: Prepubertal Oocyte Donors
Dolors Izquierdo1, María-Gracia Catalá2, María-Teresa Paramio2
1Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, Barcelona, Spain. dolors.Izquierdo@uab.cat.
Methods in Molecular Biology (Clifton, N.J.)
|June 24, 2019
Summary
Juvenile in vitro embryo technologies (JIVET) enable faster genetic gain in livestock breeding by producing embryos from young females. This method efficiently generates in vitro embryos from prepubertal sheep and goats for enhanced breeding programs.
Area of Science:
- Reproductive Biology
- Animal Science
- Biotechnology
Background:
- Juvenile in vitro embryo technologies (JIVET) are crucial for accelerating genetic gain in livestock.
- Shortening the generation interval is a key objective in animal breeding programs.
- Utilizing oocytes from prepubertal females offers a strategy to enhance breeding efficiency.
Purpose of the Study:
- To detail the methodology for producing in vitro embryos from prepubertal sheep and goats.
- To establish a reliable JIVET protocol for efficient embryo production.
- To support genetic improvement initiatives in livestock breeding.
Main Methods:
- Collection of cumulus-oocyte complexes (COCs) from ovaries of prepubertal sheep (90-120 days) and goats (30-45 days).
- In vitro maturation of COCs in TCM199 with 10% fetal bovine serum (FBS) at 38.5°C with 5% CO2 for 24 hours.
- Sperm preparation via density gradient, followed by coincubation with mature COCs and subsequent embryo culture in SOF medium for 8 days.
Main Results:
- Successful production of in vitro embryos from prepubertal sheep and goats.
- Development of embryos to the blastocyst stage within 8 days of culture.
- Demonstration of a viable protocol for JIVET in small ruminants.
Conclusions:
- The described JIVET methodology is effective for producing in vitro embryos from young sheep and goats.
- This approach can significantly contribute to increasing genetic gain in livestock breeding programs.
- JIVET offers a valuable tool for efficient animal reproduction and genetic improvement.
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