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Updated: Jan 23, 2026

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Published on: July 14, 2014
In Vitro Embryo Production in Buffalo Species (Bubalus bubalis)
Marcello Rubessa1, Lucia Boccia2, Serena Di Francesco3
1Department of Animal Sciences, University of Illinois, Urbana, IL, USA. rubessa@illinois.edu.
Abstract:
In the last years there has been a growing attention in the world regarding buffalo (Bubalus bubalis) breeding. In tropical countries, climatic and environmental conditions make buffalo an irreplaceable milk producer. Since cattle cannot express their own productive potential in these conditions, buffaloes are best suited to satisfy animal protein demand, as they are easy to maintain, long-lived, and parasite resistant. Here we describe how to produce buffalo embryos from slaughterhouse oocytes, with protocols for media formulation, oocyte selection, semen capacitation, and in the end embryo evaluation.
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