In Vitro Culture of Embryos from Domestic Cats

A variety of protocols are available for the production of feline blastocysts from oocytes that are matured and fertilized in vitro. However, most of these protocols utilize media that were developed for other cell types or the embryos of other species. The protocol outlined in this chapter is based on a series of studies that led to the development of feline optimized culture medium (FOCM), a feline-specific, sequential medium system. The initial embryo culture medium was subsequently modified for both IVM and IVF. Using a small number of stock solutions, a series of media can be easily prepared that allows for immature cat oocytes to be matured and fertilized in vitro, with the resulting embryos developing to the blastocyst stage without the use of serum.