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DNAJC2 is required for mouse early embryonic development

Louise Helary1, Johan Castille2, Bruno Passet2

  • 1UMR 1313 GABI, INRA, AgroParisTech, Université de Saclay, F-78350, Jouy-en-Josas, France; Univ. Limoges, INRA, PEIRENE EA7500, USC1061 GAMAA, F-87000, Limoges, France.

Biochemical and Biophysical Research Communications
|June 25, 2019
PubMed

Insights

The DNAJC2 protein, crucial for cell function, is essential for early mouse embryonic development. Its absence causes embryonic lethality, highlighting its vital role in embryogenesis.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • DNAJC2 protein (also known as ZRF1 or MPP11) functions as both a chaperone and a chromatin regulator.
  • Its involvement in stem cell differentiation and association with cancer malignancies are documented.
  • The specific role of the Dnajc2 gene in mouse embryogenesis remains unexplored.

Purpose of the Study:

  • To investigate the function of the Dnajc2 gene during early mouse embryogenesis.
  • To determine the consequences of Dnajc2 gene invalidation on embryonic development.

Main Methods:

  • Gene invalidation in FVB/Nj mice using the CRISPR/Cas9 system.
  • Utilizing siRNAs against Dnajc2 in mouse 1-cell embryos to assess maternal mRNA effects.

Main Results:

  • Complete invalidation of the Dnajc2 gene resulted in early post-implantation lethality of nullizygous embryos.
  • Depletion of Dnajc2 via siRNA in 1-cell embryos suggested a role for maternal Dnajc2 mRNA in preimplantation development.

Conclusions:

  • The Dnajc2 gene is essential for successful early mouse embryogenesis.
  • These findings establish DNAJC2 as a critical factor for embryonic development, with implications for understanding developmental disorders and cancer.
Keywords:
DNAJC2DevelopmentEarly embryoKnockoutMouse

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