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Mouse Embryonic Development in a Serum-free Whole Embryo Culture System
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DNAJC2 is required for mouse early embryonic development.

Louise Helary1, Johan Castille2, Bruno Passet2

  • 1UMR 1313 GABI, INRA, AgroParisTech, Université de Saclay, F-78350, Jouy-en-Josas, France; Univ. Limoges, INRA, PEIRENE EA7500, USC1061 GAMAA, F-87000, Limoges, France.

Biochemical and Biophysical Research Communications
|June 25, 2019
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Summary

The DNAJC2 protein, crucial for cell function, is essential for early mouse embryonic development. Its absence causes embryonic lethality, highlighting its vital role in embryogenesis.

Keywords:
DNAJC2DevelopmentEarly embryoKnockoutMouse

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • DNAJC2 protein (also known as ZRF1 or MPP11) functions as both a chaperone and a chromatin regulator.
  • Its involvement in stem cell differentiation and association with cancer malignancies are documented.
  • The specific role of the Dnajc2 gene in mouse embryogenesis remains unexplored.

Purpose of the Study:

  • To investigate the function of the Dnajc2 gene during early mouse embryogenesis.
  • To determine the consequences of Dnajc2 gene invalidation on embryonic development.

Main Methods:

  • Gene invalidation in FVB/Nj mice using the CRISPR/Cas9 system.
  • Utilizing siRNAs against Dnajc2 in mouse 1-cell embryos to assess maternal mRNA effects.

Main Results:

  • Complete invalidation of the Dnajc2 gene resulted in early post-implantation lethality of nullizygous embryos.
  • Depletion of Dnajc2 via siRNA in 1-cell embryos suggested a role for maternal Dnajc2 mRNA in preimplantation development.

Conclusions:

  • The Dnajc2 gene is essential for successful early mouse embryogenesis.
  • These findings establish DNAJC2 as a critical factor for embryonic development, with implications for understanding developmental disorders and cancer.