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Dynamic Podosome-Like Structures in Nascent Phagosomes Are Coordinated by Phosphoinositides
Philip P Ostrowski1, Spencer A Freeman2, Gregory Fairn3
1Program in Cell Biology, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; Department of Biochemistry, University of Toronto, Toronto, ON M5S 1A8, Canada.
Developmental Cell
|June 25, 2019
Summary
Phagocytosis involves dynamic F-actin structures. New podosome-like structures form and expand during particle engulfment, aiding receptor activation and membrane bending.
Area of Science:
- Cell Biology
- Immunology
Background:
- Phagocytosis, a critical cellular process, relies on F-actin polymerization.
- The precise nature and dynamics of F-actin structures during phagocytosis remain unclear.
Purpose of the Study:
- To investigate the F-actin structures formed during Fc receptor-mediated phagocytosis.
- To elucidate the dynamics and function of these structures in target engulfment.
Main Methods:
- Super-resolution microscopy
- Interference reflection microscopy
Main Results:
- Observed formation of short-lived, radially expanding podosome-like structures during phagocytosis.
- These structures require localized phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3) formation.
- Podosome-like structures disappear centrally, facilitated by phosphatidylinositol (4,5)-bisphosphate (PtdIns(4,5)P2) hydrolysis, enabling membrane bending.
- Structures attach to targets, promoting receptor engagement and lamellipodia extension.
Conclusions:
- Podosome-like structures are dynamic and essential for efficient phagocytosis.
- Their formation, expansion, and regulated disappearance facilitate target engulfment and cellular response.
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