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While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
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Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
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Bartonella gene transfer agent: Evolution, function, and proposed role in host adaptation.

Maxime Québatte1, Christoph Dehio1

  • 1Biozentrum, University of Basel, Basel, Switzerland.

Cellular Microbiology
|June 25, 2019
PubMed
Summary

Bartonella bacteria adapt to new hosts via horizontal gene transfer (HGT) mediated by the Bartonella gene transfer agent (BaGTA). BaGTA drives evolution and combats harmful mutations in these zoonotic pathogens.

Keywords:
BartonellaMuller's ratchetgene transfer agent (GTA)horizontal gene transfer (HGT)host adaptationtype IV secretion systems (T4SS)

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Area of Science:

  • Microbiology
  • Evolutionary Biology
  • Pathogen Ecology

Background:

  • Bacterial pathogens like Bartonella exhibit remarkable host adaptability, crucial for their clinical, ecological, and evolutionary impact.
  • Bartonella diversification into multiple species is linked to adaptations for a facultative intracellular lifestyle and colonization of diverse mammalian hosts.
  • Horizontal gene transfer (HGT) and recombination within a limited pan-genome are hypothesized drivers of Bartonella's host adaptability.

Purpose of the Study:

  • To review recent advancements in understanding the evolution, function, and impact of the Bartonella gene transfer agent (BaGTA).
  • To explore BaGTA's role in driving genome diversification and facilitating host adaptability in Bartonella species.
  • To discuss BaGTA's potential role in mitigating Muller's ratchet (accumulation of deleterious mutations) during Bartonella's complex infection cycles.

Main Methods:

  • Review of recent functional and evolutionary studies on Bartonella spp.
  • Analysis of the role of the Bartonella gene transfer agent (BaGTA) in mediating horizontal gene transfer (HGT).
  • Discussion of genomic and evolutionary mechanisms contributing to pathogen adaptation and diversification.

Main Results:

  • The Bartonella gene transfer agent (BaGTA) is identified as a key mediator of highly efficient HGT in Bartonella.
  • BaGTA likely contributes significantly to genome diversification by enabling recombination of advantageous traits for host adaptation.
  • BaGTA may counteract Muller's ratchet, preventing the accumulation of deleterious mutations in Bartonella populations.

Conclusions:

  • BaGTA is a critical factor in the adaptive radiation and host specialization of Bartonella pathogens.
  • Understanding BaGTA's function provides insights into bacterial evolution, pathogenesis, and zoonotic disease dynamics.
  • BaGTA's role in genome maintenance and diversification is central to the success of Bartonella spp. in their ecological niches.