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Related Concept Videos

Chirality02:25

Chirality

29.2K
Chirality is a term that describes the lack of mirror symmetry in an object. In other words, chiral objects cannot be superposed on their mirror images. For example, our feet are chiral, as the mirror image of the left foot, the right foot, cannot be superposed on the left foot.
Chiral objects exhibit a sense of handedness when they interact with another chiral object. For example, our left foot can only fit in the left shoe and not in the right shoe. Achiral objects — objects that have...
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Chirality in Nature02:30

Chirality in Nature

16.9K
Chirality is the most intriguing yet essential facet of nature, governing life’s biochemical processes and precision. It can be observed from a snail shell pattern in a macroscopic world to an amino acid, the minutest building block of life. Most of the snails around the world have right-coiled shells because of the intrinsic chirality in their genes. All the amino acids present in the human body exist in an enantiomerically pure state, except for glycine - the sole achiral amino acid.
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Peptide Bonds02:43

Peptide Bonds

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A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
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Molecules with Multiple Chiral Centers02:25

Molecules with Multiple Chiral Centers

14.8K
Molecules that possess multiple chiral centers can afford a large number of stereoisomers. For instance, while some molecules like 2-butanol have one chiral center, defined as a tetrahedral carbon atom with four different substituents attached, several molecules like butane-2,3-diol have multiple chiral centers. A simple formula to predict the number of stereoisomers possible for a molecule with n chiral centers is 2n. However, there can be a lower number where some of the stereoisomers are...
14.8K
Chirality at Nitrogen, Phosphorus, and Sulfur02:30

Chirality at Nitrogen, Phosphorus, and Sulfur

6.9K
Chirality is most prevalent in carbon-based tetrahedral compounds, but this important facet of molecular symmetry extends to sp3-hybridized nitrogen, phosphorus and sulfur centers, including trivalent molecules with lone pairs. Here, the lone pair behaves as a functional group in addition to the other three substituents to form an analogous tetrahedral center that can be chiral.
A consequence of chirality is the need for enantiomeric resolution. While this is theoretically possible for all...
6.9K
Protein Complex Assembly02:41

Protein Complex Assembly

16.7K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
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Related Experiment Video

Updated: Jan 23, 2026

Generation and Characterization of Rat Uterus Organoids from Rat Endometrial Epithelial Stem Cells
05:12

Generation and Characterization of Rat Uterus Organoids from Rat Endometrial Epithelial Stem Cells

Published on: August 2, 2024

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[Chirality Self-assembling peptide for rats endometrial regeneration model].

Shuyi Wu1, Shijian Lan1, Jing Wen1

  • 1College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|June 25, 2019
PubMed
Summary

Chiral self-assembling peptides form stable structures and promote uterine tissue repair by reducing inflammation and enhancing blood vessel growth. These peptides offer a promising scaffold for 3D cell culture and regenerative medicine applications.

Keywords:
Chiral Self-assembling peptiderats modeluterine repair

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Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates
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Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates

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Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
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Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

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Related Experiment Videos

Last Updated: Jan 23, 2026

Generation and Characterization of Rat Uterus Organoids from Rat Endometrial Epithelial Stem Cells
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Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates
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Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
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Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Peptide Chemistry

Background:

  • Chiral self-assembling peptides (CSPs) are emerging as promising biomaterials.
  • Their potential in tissue regeneration, particularly for uterine trauma, requires further investigation.
  • Understanding their physical, chemical, and biological properties is crucial for therapeutic development.

Purpose of the Study:

  • To investigate the physical and chemical properties of CSPs.
  • To evaluate the efficacy of CSPs in promoting uterine trauma regeneration.
  • To explore CSPs as a scaffold for three-dimensional (3D) cell culture.

Main Methods:

  • Circular dichroism and Congo red staining were used to analyze peptide self-assembly and secondary structure.
  • Erythrocyte lysis assay assessed peptide interaction with cell membranes.
  • Cell viability was compared in 2D and 3D cultures using CCK-8 assay.
  • A rat endometrium curettage model was used with HE and immunohistochemistry staining to evaluate regenerative effects.

Main Results:

  • CSPs exhibited a stable beta-sheet secondary structure and formed dense membrane structures within 24 hours in salt ions.
  • Peptides showed no cytotoxicity to cell membranes.
  • In vivo studies demonstrated that CSPs significantly reduced inflammation, promoted neovascularization, and accelerated uterine tissue repair.
  • 3D cultures using CSP nanofiber scaffolds supported cell growth.

Conclusions:

  • Chiral self-assembling peptides possess favorable physical and chemical properties for biomaterial applications.
  • CSPs effectively promote uterine trauma regeneration by modulating inflammatory responses and enhancing vascularization.
  • These peptides serve as a novel scaffold material for 3D cell culture and hold significant potential for uterine repair.