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Estimation of Plant Biomass Lignin Content using Thioglycolic Acid TGA
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Estimating belowground plant abundance with DNA metabarcoding.

Silvia Matesanz1, David S Pescador1, Beatriz Pías2

  • 1Área de Biodiversidad y Conservación, Universidad Rey Juan Carlos, Móstoles, Spain.

Molecular Ecology Resources
|June 25, 2019
PubMed
Summary

DNA metabarcoding can now accurately quantify plant root biomass. Correction indices significantly improved abundance estimates in mixtures, enabling better study of belowground plant ecology.

Keywords:
DNA metabarcodingMediterranean shrublandcoexistencemock communitiesplant abundancerbcL regionroot biomasssequence

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Area of Science:

  • Plant Ecology
  • Molecular Ecology
  • Bioinformatics

Background:

  • Plant community ecology traditionally focuses on aboveground biomass, overlooking crucial belowground processes.
  • DNA metabarcoding offers a solution to logistical challenges in sampling belowground plant communities.
  • Quantifying species abundance using sequence percentages in DNA metabarcoding remains a significant limitation.

Purpose of the Study:

  • To develop and validate methods for accurate quantification of root biomass using DNA metabarcoding.
  • To improve estimates of species' relative abundance in belowground plant communities.
  • To enable robust ecological studies of belowground plant community dynamics.

Main Methods:

  • Construction of pairwise and multispecies root tissue mixtures of known biomass proportions.
  • Application of linear model fits and correction indices to DNA metabarcoding sequence data.
  • Validation of quantification methods using mock communities simulating field samples.

Main Results:

  • A significant positive correlation was observed between sequence percentage and biomass for all species (R² = .44–.66).
  • Species-specific differences in slope and intercept highlighted the need for correction methods.
  • Correction indices reduced overall biomass estimation error from 17% to 6% in multispecies mixtures.

Conclusions:

  • Post-sequencing quantification methods, particularly correction indices, enhance DNA metabarcoding's utility for determining root biomass and relative abundance.
  • Accurate belowground biomass quantification is crucial for understanding plant community ecology.
  • This methodology advances the study of vital, yet under-researched, belowground ecological processes.