Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Packaging02:21

Chromatin Packaging

21.8K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
21.8K
Chromatin Packaging01:32

Chromatin Packaging

19.0K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
19.0K
Chromatin Packaging02:21

Chromatin Packaging

9.6K
9.6K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.4K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.4K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

7.3K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
7.3K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

24.7K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

KAS-CUT&Tag for direct mapping of transcription bubbles.

bioRxiv : the preprint server for biology·2026
Same author

Isoform-specific cofactor recruitment through the intrinsically disordered N-terminus of p63 underlies differential transcriptional activities.

bioRxiv : the preprint server for biology·2026
Same author

Superabundant microRNAs are transcribed from human rDNA spacer promoters insulated by CTCF.

Science advances·2026
Same author

BACH2 regulates T cell lineage state to enhance CAR T cell function.

Nature immunology·2026
Same author

Cell-cycle-dependent repression of histone gene transcription by histone H4.

Nature structural & molecular biology·2026
Same author

An integrated view of the structure and function of the human 4D nucleome.

Nature·2025
Same journal

Arrayed single-gene perturbations identify drivers of human anterior neural tube closure.

eLife·2026
Same journal

Pervasive relaxed selection on spermatogenesis genes coincident with the evolution of polygyny in gorillas.

eLife·2026
Same journal

Impacts of DNA methylation on H2A.Z deposition and nucleosome stability.

eLife·2026
Same journal

Continuous developmental changes in word recognition and language learning across early childhood.

eLife·2026
Same journal

Multiple event segmentation mechanisms in the human brain.

eLife·2026
Same journal

Optimised genome editing for precise DNA insertion and substitution using prime editors in zebrafish.

eLife·2026
See all related articles

Related Experiment Video

Updated: Jan 23, 2026

Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle
09:30

Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle

Published on: November 6, 2017

9.1K

Improved CUT&RUN chromatin profiling tools.

Michael P Meers1, Terri D Bryson1,2, Jorja G Henikoff1

  • 1Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, United States.

Elife
|June 25, 2019
PubMed
Summary
This summary is machine-generated.

Chromatin, Immunoprecipitation, CUT&RUN, epigenomic profiling. We enhanced CUT&RUN (Cleavage Under Targets and Release помощью Nuclease) with new features for broader antibody compatibility and improved accuracy. This makes it ideal for routine epigenomic profiling.

Keywords:
chromatinchromosomesepigenomicsgene expressiongeneticsgenomicshumanspike-in calibration

More Related Videos

Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples
09:38

Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples

Published on: November 23, 2016

19.9K
Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
07:41

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Published on: October 2, 2017

8.8K

Related Experiment Videos

Last Updated: Jan 23, 2026

Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle
09:30

Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle

Published on: November 6, 2017

9.1K
Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples
09:38

Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples

Published on: November 23, 2016

19.9K
Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
07:41

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Published on: October 2, 2017

8.8K

Area of Science:

  • Molecular Biology
  • Genomics
  • Epigenetics

Background:

  • Chromatin Immunoprecipitation (ChIP) is a common method for epigenomic profiling.
  • CUT&RUN (Cleavage Under Targets and Release помощью Nuclease) offers an alternative to ChIP.
  • Previous CUT&RUN utilized a Protein A-Micrococcal Nuclease (pA/MNase) fusion protein.

Purpose of the Study:

  • To introduce significant enhancements to the CUT&RUN technique.
  • To expand antibody compatibility and simplify purification processes.
  • To improve the accuracy and reliability of epigenomic profiling.

Main Methods:

  • Development of a hybrid Protein A-Protein G-MNase construct.
  • Implementation of a modified digestion protocol to prevent premature complex release.
  • Introduction of a calibration strategy using *E. coli* DNA carry-over.

Main Results:

  • The enhanced CUT&RUN method demonstrates broader antibody compatibility.
  • The modified protocol ensures stability of the nuclease-bound complex.
  • The calibration strategy allows for more accurate data normalization.

Conclusions:

  • The improved CUT&RUN technique offers a low-cost, efficient, and reproducible method for epigenomic profiling.
  • These enhancements position CUT&RUN as a preferred method for routine epigenomic analysis.
  • The study highlights the versatility and robustness of the CUT&RUN assay.