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High-Throughput Analysis of Optical Mapping Data Using ElectroMap
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High-Throughput Analysis of Optical Mapping Data Using ElectroMap.

Christopher O'Shea1, Andrew P Holmes2, Ting Y Yu3

  • 1Institute of Cardiovascular Sciences, University of Birmingham; EPSRC Centre for Doctoral Training in Physical Sciences for Health, School of Chemistry, University of Birmingham; School of Computer Science, University of Birmingham.

Journal of Visualized Experiments : Jove
|June 25, 2019
PubMed
Summary
This summary is machine-generated.

ElectroMap software provides a comprehensive tool for analyzing cardiac electrophysiology data from optical mapping. It enables high-throughput analysis of voltage and calcium datasets, accelerating research in cardiac function.

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Area of Science:

  • Cardiovascular Research
  • Biophysics
  • Computational Biology

Background:

  • Optical mapping is crucial for studying cardiac electrophysiology at high resolution.
  • Analyzing complex optical mapping data requires specialized software tools.

Purpose of the Study:

  • To present a step-by-step guide for using ElectroMap software.
  • To demonstrate ElectroMap's capabilities for analyzing cardiac voltage and calcium datasets.

Main Methods:

  • Utilized ElectroMap software for data analysis and visualization.
  • Employed built-in functions for pacing frequency detection, signal segmentation, and multi-beat averaging.
  • Applied custom modules for detailed analysis of conduction, single file propagation, and alternans.

Main Results:

  • ElectroMap facilitates high-throughput analysis of optical mapping data.
  • Automated features improve signal quality and enable detection of subtle electrophysiological changes.
  • Custom modules allow in-depth investigation of specific cardiac electrophysiology phenomena.

Conclusions:

  • ElectroMap is a versatile software platform for processing and analyzing complex cardiac electrophysiology data.
  • The software accelerates research by enabling efficient and detailed analysis of optical mapping experiments.