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Updated: Jan 23, 2026

Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation
Published on: June 7, 2019
Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation
Martin Tran1, Mohit Dave2, Rusty Lansford3
1Department of Biological Sciences, University of Southern California, Los Angeles.
None:
We report that mRNA electroporation permits fluorescent proteins to label cells in living quail embryos more quickly and broadly than DNA electroporation. The high transfection efficiency permits at least 4 distinct mRNAs to be co-transfected with ~87% efficiency. Most of the electroporated mRNAs are degraded during the first 2 h post-electroporation, permitting time-sensitive experiments to be carried out in the developing embryo. Finally, we describe how to dynamically image live embryos electroporated with mRNAs that encode various subcellular targeted fluorescent proteins.
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